Caspase-3 induced apoptosis in transgenic zebrafish (Retracted Article. See vol 29, pg 843, 2007)
Caspase-3 induced apoptosis in transgenic zebrafish (Retracted Article. See vol 29, pg 843, 2007)
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DOI：
10.1007/s10529-005-5334-5
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发表时间：
2006-02-01
影响因子：
2.7
通讯作者：
Sadhu, AK
中科院分区：
文献类型：
--
作者：
Chakraborty, C;Saha, G;Sadhu, AK
Zebrafish is an attractive model organism for studying apoptosis development because of its genetic accessibility. Here we describe the induction of clonally derived apoptosis in transgenic zebrafish expressing mouse caspase-3 (CASP3) under control of the zebrafish beta-actin promoter (beta p). Visualization of apoptotic cells, expressing a chimeric transgene encoding CASP3 fused to green fluorescent protein (GFP) gene, revealed that apoptosis arose in the thymus, spread locally into gill arches and retro-orbital soft tissue, and then disseminated into abdominal organs like testis, kidney. This transgenic model provides a platform for over-expression of caspase-3 induced extensive apoptosis in embryos and adult.
