Synthetic Switches and Regulatory Circuits in Plants
Synthetic Switches and Regulatory Circuits in Plants
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DOI：
10.1104/pp.18.01362
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发表时间：
2019-03-01
期刊：
影响因子：
7.4
通讯作者：
Zurbriggen, Matias D.
中科院分区：
文献类型：
--
作者：
Andres, Jennifer;Blomeier, Tim;Zurbriggen, Matias D.
合成生物学是一个成熟但不断发展的跨学科研究领域，目前正在彻底改变生物医学研究和生物技术产业。在细菌、酵母和动物系统中合成电路的工程化促进了对遗传和代谢网络的理解和操纵的相当大的进步;然而，它们在植物领域的实施滞后。在这里，我们回顾了合成化学和光调控（光遗传学）开关工程的理论实验方法，用于细胞过程的有针对性的询问和控制，包括在植物领域的现有应用。我们强调的策略，为不同复杂性的合成电路的遗传部件的模块化组装，从布尔逻辑门和振荡器件到半和全合成开环和闭环分子和细胞电路。最后，我们探讨了这些方法在植物新功能工程中的潜在应用，包括了解复杂的信号网络，提高作物产量和生产生物药物。
Synthetic biology is an established but ever-growing interdisciplinary field of research currently revolutionizing biomedicine studies and the biotech industry. The engineering of synthetic circuitry in bacterial, yeast, and animal systems prompted considerable advances for the understanding and manipulation of genetic and metabolic networks; however, their implementation in the plant field lags behind. Here, we review theoretical-experimental approaches to the engineering of synthetic chemical-and light-regulated (optogenetic) switches for the targeted interrogation and control of cellular processes, including existing applications in the plant field. We highlight the strategies for the modular assembly of genetic parts into synthetic circuits of different complexity, ranging from Boolean logic gates and oscillatory devices up to semi-and fully synthetic open-and closed-loop molecular and cellular circuits. Finally, we explore potential applications of these approaches for the engineering of novel functionalities in plants, including understanding complex signaling networks, improving crop productivity, and the production of biopharmaceuticals.