Preparation of Well-Defined Polymers and DNA–Polymer Bioconjugates via Small-Volume eATRP in the Presence of Air
Preparation of Well-Defined Polymers and DNA–Polymer Bioconjugates via Small-Volume eATRP in the Presence of Air
复制标题
在空气存在下通过小体积 eATRP 制备结构明确的聚合物和 DNA 聚合物生物缀合物
DOI：
10.1021/acsmacrolett.9b00159
复制
发表时间：
2019
影响因子：
7.015
通讯作者：
Matyjaszewski, Krzysztof
中科院分区：
文献类型：
--
作者：
Sun, Yue;Lathwal, Sushil;Wang, Yi;Fu, Liye;Olszewski, Mateusz;Fantin, Marco;Enciso, Alan E.;Szczepaniak, Grzegorz;Das, Subha;Matyjaszewski, Krzysztof
在沉积在丝网印刷电极 (SPE) 上的小体积溶液 (75 μL) 中进行水性电化学介导的原子转移自由基聚合 (eATRP)。由于使用葡萄糖氧化酶（GOx）作为除氧剂，反应是在空气中进行的。通过小体积 eATRP 在室温下合成了结构明确的聚（2-（甲基亚磺酰基）乙基丙烯酸酯）（PMSEA）、聚（低聚（环氧乙烷）甲基醚甲基丙烯酸酯）（POEOMA）以及相应的 DNA-聚合物生物杂化物。通过应用酶脱氧系统和紧凑的电化学装置，简化了反应并提高了聚合速率。重要的是，聚合混合物的体积降低至微升，这不仅降低了每个反应的成本，而且还可以在高通量系统的组合化学和电极阵列配置中实现。
An aqueous electrochemically mediated atom transfer radical polymerization (eATRP) was performed in a small volume solution (75 μL) deposited on a screen-printed electrode (SPE). The reaction was open to air, thanks to the use of glucose oxidase (GOx) as an oxygen scavenger. Well-defined poly(2-(methylsulfinyl)ethyl acrylate) (PMSEA), poly(oligo(ethylene oxide) methyl ether methacrylate) (POEOMA), and corresponding DNA–polymer biohybrids were synthesized by the small-volume eATRP at room temperature. The reactions were simplified and polymerization rates increased by the application of the enzyme deoxygenating system and the compact electrochemical setup. Importantly, the volume of polymerization mixture was lowered to microliters, which not only decreases the cost for each reaction, but can also be potentially implemented in combinatorial chemistry and electrode-array configurations for high-throughput systems.