Genomic and Transcriptomic Associations Identify a New Insecticide Resistance Phenotype for the Selective Sweep at the Cyp6g1 Locus of Drosophila melanogaster.
Genomic and Transcriptomic Associations Identify a New Insecticide Resistance Phenotype for the Selective Sweep at the Cyp6g1 Locus of Drosophila melanogaster.
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DOI：
10.1534/g3.116.031054
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发表时间：
2016-08-09
期刊：
影响因子：
--
通讯作者：
Robin C
中科院分区：
文献类型：
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作者：
Battlay P;Schmidt JM;Fournier-Level A;Robin C
对果蝇基因组的扫描已经在那些具有最明显的正选择特征的位点中鉴定出了有机磷抗性位点。在这项研究中，使用果蝇遗传参考面板和全基因组关联研究了对有机磷杀虫剂甲基嗪草酮抗性的分子基础。最近发布的全转录组数据被用于扩展果蝇遗传参考小组资源的效用，超越传统的全基因组关联研究，以允许表型的系统遗传学分析。我们发现，基因组和转录组学关联独立地确定Cyp 6 g1，一个基因参与滴滴涕和烟碱类杀虫剂的抗性，作为最佳候选人的azinoprotein-甲基抗性。这通过使用来自抗性等位基因的天然调控元件转基因过表达Cyp 6 g1来验证，导致抗性增加6.5倍。我们还确定了四个新的候选基因与azinid-methyl抗性，所有这些都参与调节脂肪储存，或神经系统发育。在Cyp 6 g1中，我们发现了一个可证明的抗性位点，验证了转录组数据可用于识别与杀虫剂抗性相关的变体，以及全基因组关联研究和全基因组选择性扫描分析的峰之间的重叠。
Scans of the Drosophila melanogaster genome have identified organophosphate resistance loci among those with the most pronounced signature of positive selection. In this study, the molecular basis of resistance to the organophosphate insecticide azinphos-methyl was investigated using the Drosophila Genetic Reference Panel, and genome-wide association. Recently released full transcriptome data were used to extend the utility of the Drosophila Genetic Reference Panel resource beyond traditional genome-wide association studies to allow systems genetics analyses of phenotypes. We found that both genomic and transcriptomic associations independently identified Cyp6g1, a gene involved in resistance to DDT and neonicotinoid insecticides, as the top candidate for azinphos-methyl resistance. This was verified by transgenically overexpressing Cyp6g1 using natural regulatory elements from a resistant allele, resulting in a 6.5-fold increase in resistance. We also identified four novel candidate genes associated with azinphos-methyl resistance, all of which are involved in either regulation of fat storage, or nervous system development. In Cyp6g1, we find a demonstrable resistance locus, a verification that transcriptome data can be used to identify variants associated with insecticide resistance, and an overlap between peaks of a genome-wide association study, and a genome-wide selective sweep analysis.