Increased efficiency of fluorescence in situ hybridization (FISH) using the microwave
Increased efficiency of fluorescence in situ hybridization (FISH) using the microwave
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DOI：
10.1369/jhc.5b6632.2005
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发表时间：
2005-10-01
影响因子：
3.2
通讯作者：
Halbhuber, KJ
中科院分区：
文献类型：
--
作者：
Weise, A;Liehr, T;Halbhuber, KJ
本文介绍了一种新的荧光原位杂交方法。FISH信号通过在DNA-DNA杂交过程中施加的微波脉冲增强。这是第一次描述通过应用微波更有效地进行单/低拷贝探针的FISH;后者导致快速结果或增强弱信号。微波FISH与常规FISH进行了系统的比较，我们可以证明微波辐射的效率，特别是在杂交的前100分钟。
A new method is described for performing fluorescence in situ hybridization (FISH). FISH signals are enhanced by microwave pulses applied during the DNA-DNA hybridization process. it is the first description of FISH with a single/low-copy probe done more efficiently by application of microwave; the latter leads to quick results or enhancement of weak signals. Microwave FISH has been compared systematically with normal FISH, and we could demonstrate the efficiency of microwave irradiation especially in the first 100 min of hybridization.