Molecular mechanisms of cardioprotection by taurine on ischemia-induced apoptosis in cultured cardiomyocytes.
Molecular mechanisms of cardioprotection by taurine on ischemia-induced apoptosis in cultured cardiomyocytes.
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DOI：
10.1007/978-0-387-33504-9_28
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发表时间：
2006
影响因子：
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通讯作者：
Kyoko Takahashi;T. Takatani;Y. Uozumi;Takashi Ito;T. Matsuda;Y. Fujio;S. Schaffer;J. Azuma
中科院分区：
文献类型：
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作者：
Kyoko Takahashi;T. Takatani;Y. Uozumi;Takashi Ito;T. Matsuda;Y. Fujio;S. Schaffer;J. Azuma
心力衰竭发病机制的一个组成部分是心肌细胞丢失。心肌细胞损失的传统解释是细胞坏死，但大量证据证实细胞凋亡在心力衰竭发生中的作用（Garg 等，2005）。心肌梗塞的临床病例以及连续缺血或缺血/再灌注的兔、大鼠和小鼠模型中都显示了细胞凋亡的证据（Garg 等，2005）。研究表明，线粒体途径参与缺血诱导的细胞凋亡（Garg et al., 2005）。牛磺酸（2-氨基乙磺酸），即氨基酸，是调节缺血期间细胞凋亡程度的因素之一（Roysommuti 等，2003；Schaffer 等，2003）。然而，人们对介导这种反应的细胞保护信号通路知之甚少。我们之前曾报道，当暴露于含有 20 mM 牛磺酸的培养基时，分离的新生儿心肌细胞对缺血诱导的细胞凋亡具有抵抗力（Takahashi 等人，2003）。在这项研究中，在新开发的模拟缺血模型中研究了牛磺酸和线粒体介导的细胞凋亡之间的相互作用，该模型利用分离的心肌细胞，将其与含有和缺乏牛磺酸的培养基一起孵育，然后密封在培养瓶中（Takahashi et al., 2003; Takatani et al., 2004a, b）。
An integral part of the pathogenesis of heart failure is myocyte loss. The traditional explanation for myocyte loss was cell necrosis but there has been a surge of evidence affirming the role of apoptosis in the genesis of heart failure (Garg et al., 2005). Evidence for apoptotic cell death was shown in clinical cases of myocardial infarction, as well as in rabbit, rat, and mouse models of continuous ischemia or ischemia/reperfusion (Garg et al., 2005). It has been shown that the mitochondrial pathways participate in apoptosis induced by ischemia (Garg et al., 2005). Taurine (2-aminoethanesulfonic acid), the-amino acid, is one of the factors that regulates the degree of apoptosis during ischemia (Roysommuti et al., 2003; Schaffer et al., 2003). However, little is known about the cytoprotective signalling pathways mediate this response. We have previously reported that isolated neonatal cardiomyocytes become resistant to ischemia-induced apoptosis when exposed to medium containing 20 mM taurine (Takahashi et al., 2003). In this study, the interaction between taurine and mitochondria-mediated apoptosis is investigated in a newly developed simulated ischemia model utilizing isolated cardiomyocytes that are incubated with medium containing and lacking taurine and then sealed within cultured flasks (Takahashi et al., 2003, Takatani et al., 2004a, b).