Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.
Size-dependent cellular toxicity and uptake of commercial colloidal gold nanoparticles in DU-145 cells.
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DOI：
10.1007/s12645-013-0033-8
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发表时间：
2013
影响因子：
5.7
通讯作者：
Tzeng TR
中科院分区：
文献类型：
--
作者：
Vedantam P;Huang G;Tzeng TR
尿路感染(UTI)是前列腺癌患者的主要疾病。大肠杆菌ORN178(EC-178)是一种通过与前列腺癌细胞(DU-145细胞)的粘附素特异性结合而导致反复感染的尿路病原体。金纳米颗粒(GNPs)已被用于病原体的生物诊断。在这项研究中，我们研究了EC-178与DU-145细胞的结合时间，普通和甘露糖功能化的细胞毒性和摄取，以及20和200 nm的GNPs(d-甘露聚糖(Mn)-GNPs)。我们还研究了GNPs与胎牛血清孵育时的蛋白质冠层，以研究决定GNPs生物学命运的蛋白质冠层。孵育3小时后，EC-178与DU-145细胞结合并进入DU-145细胞内。平坦的20 nm GNPs可降低DU-145细胞对数生长期和滞后期48h和72h的细胞存活率。DU-145细胞对Mn-GNPs的摄取显著高于普通GNPs。动态光散射法和SDS-PAGE凝胶分析证实，GNPs与胎牛血清孵育后可观察到蛋白质日冕。
Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.