RESPONSES TO BORDETELLA-PERTUSSIS MUTANT STRAINS AND TO VACCINATION IN THE COUGHING VAT MODEL OF PERTUSSIS
RESPONSES TO BORDETELLA-PERTUSSIS MUTANT STRAINS AND TO VACCINATION IN THE COUGHING VAT MODEL OF PERTUSSIS
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DOI：
10.1099/00222615-40-5-307
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发表时间：
1994-05-01
影响因子：
3
通讯作者：
WARDLAW, AC
中科院分区：
文献类型：
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作者：
PARTON, R;HALL, E;WARDLAW, AC
百日咳杆菌I期菌株18-323、Tohama和L-84被包裹在琼脂糖珠中并经支气管内注射给成年SD大鼠后，产生阵发性咳嗽。相反，菌株L-84的第四相变异株没有诱导咳嗽的活性，菌株BP357也是如此，BP357是一种只缺乏百日咳毒素(PT)的转座子插入突变株。BPM 1809菌株只缺乏不耐热毒素，与未修饰的I期菌株具有相似的引咳作用，表明该毒素不需要诱导咳嗽。2、百日咳对咳嗽的诱导作用也不明显。这些结果表明，百日咳杆菌第I相菌株中存在PT，而在第IV相菌株BP357和副咳杆菌中不存在PT，在百日咳大鼠模型中诱导阵发性咳嗽是必要的。预先注射DTP(全细胞)疫苗极大地降低了随后感染I-B期百日咳的大鼠的咳嗽发生率。在不同细菌株经支气管内感染后，以及接种和攻击后，监测血清学反应。通过在恢复期血清中适当存在或不存在抗PT Ig G来确认体内菌株的PT阳性或阴性状态。
Phase I strains 18-323, Tohama and L-84 of Bordetella pertussis produced paroxysmal coughing when encased in agarose beads and administered intrabronchially to adult Sprague-Dawley rats. In contrast, the Phase IV variant of strain L-84 was inactive in cough induction, as was strain BP 357, a transposon-insertion mutant which is deficient only in pertussis toxin (PT). Strain BPM 1809, which lacks only the heat-labile toxin, was similar to the unmodified Phase I strains for cough induction, indicating that this toxin is not needed to induce coughing. B, parapertussis also was inactive as a cough inducer. These results indicate that PT, present in Phase I strains of B. pertussis, and absent from Phase IV strains, strain BP 357 and B. parapertussis, is essential for the induction of paroxysmal coughing in this rat model of whooping cough. Prior injection of DTP (whole-cell) Vaccine greatly reduced the incidence of coughing in rats challenged subsequently with Phase I B. pertussis. Serological responses were monitored after intrabronchial infection with the various bacterial strains and after vaccination and challenge. The PT-positive or -negative status of the strains in vivo was confirmed by the appropriate presence or absence of anti-PT IgG in the convalescent sera.