Detection of telomerase activity and expression of TERT gene in antler of sika deer.
Detection of telomerase activity and expression of TERT gene in antler of sika deer.
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梅花鹿鹿角端粒酶活性及TERT基因表达检测
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Zhang Yu
中科院分区：
文献类型：
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作者：
Sun Haoran;Zheng Wei;Wu Shan;W. Chunyan;Zheng Mei;Tang Dan;Zhu Qiankun;Zhang Yu
[目的]观察西卡鹿茸生长发育过程中端粒酶的表达，为揭示鹿茸发育和再生机制提供新的线索。[方法]采用更新的TRAP（端粒重复序列扩增协议）银染法检测生长期鹿角尖间充质、前软骨、软骨和骨化组织中的端粒酶活性，并采用逆转录聚合酶链反应（RT-PCR）检测这些细胞中端粒酶逆转录酶（TERT）的表达。[结果]间质、软骨前体和软骨组织中均检测到端粒酶活性，而软骨组织中未检测到端粒酶活性，间质、软骨前体和软骨组织中端粒酶活性的相对值分别为91.2、46.4和13.7，此外，端粒酶基因的表达结果也支持端粒酶活性的检测结果。[结论]鹿茸生长过程中，鹿茸增殖区有端粒酶的表达，随着鹿茸细胞的进一步分化，其活性逐渐降低，端粒酶可能在鹿茸的发育和再生过程中发挥重要作用。
[Objective] To observe the expression of telomerase in antler of sika deer during growth stage and thus to provide a new line for revealing the mechanisms of development and regeneration of antlers.[Method] The updated TRAP (Telomeric repeat amplification protocol) argentum-dying method was used to detect the activity of telomerase in mesenchyme,precartilage,cartilage and ossification tissue of antler tip during the growth stage.Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the expression of telomerase reverse transcriptase (TERT) in these cells.[Result] The activity of telomerase was detected in the mesenchyme,precartilage and cartilage but not in the sclerotic tissue.The relative telomerase activity in the mesenchyme,precartilage and cartilage was 91.2,46.4 and 13.7,respectively.In addition,the results of the expression of TERT gene also supported the detection result of telomerase activity.[Conclusion] During growth phase,telomerase is expressed in multiplication area of deer antlers,and its activity decreases gradually with further differentiation of cells in antlers.Therefore,telomerase may play a key role in development and regeneration of antlers.