Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters.
Molecular Detection of Persistent Francisella tularensis Subspecies holarctica in Natural Waters.
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DOI：
10.1155/2011/851946
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发表时间：
2011-01-01
影响因子：
3.4
通讯作者：
Forsman, M
中科院分区：
文献类型：
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作者：
Broman, T;Thelaus, J;Forsman, M
土拉菌病是由土拉弗朗西斯菌（Francisella tularensis）引起的，其中F.在瑞典北方的部分地区，土拉热亚种holarctica长期以来一直是地方病的原因。尽管如此，我们对生物体自然生命周期的理解仍然有限。在三年中，我们收集了地表水样品（n = 341）和沉积物样品（n = 245）在瑞典的地方性土拉菌病的两个地区。实时荧光PCR检测表明F.土拉热菌lpnA序列分别在108个（32%）和48个（20%）样品中检测到。16SrRNA序列分析表明，这些样品均属于F.土拉热。对来自选定采样点的lpnA阳性样品的FtM19InDel区域的分析证实了F.土拉热亚种全北极特有序列。这些序列在暴发和非暴发年份的水样中检测到。我们的研究结果表明，不同的F。土拉菌样生物，包括F.土拉热亚种holarctica，在调查的地方性土拉菌病地区的自然沃茨和沉积物中持续存在。
Tularemia, caused by the bacterium Francisella tularensis, where F. tularensis subspecies holarctica has long been the cause of endemic disease in parts of northern Sweden. Despite this, our understanding of the natural life-cycle of the organism is still limited. During three years, we collected surface water samples (n = 341) and sediment samples (n = 245) in two areas in Sweden with endemic tularemia. Real-time PCR screening demonstrated the presence of F. tularenis lpnA sequences in 108 (32%) and 48 (20%) of the samples, respectively. The 16S rRNA sequences from those samples all grouped to the species F. tularensis. Analysis of the FtM19InDel region of lpnA-positive samples from selected sampling points confirmed the presence of F. tularensis subspecies holarctica-specific sequences. These sequences were detected in water sampled during both outbreak and nonoutbreak years. Our results indicate that diverse F. tularensis-like organisms, including F. tularensis subsp. holarctica, persist in natural waters and sediments in the investigated areas with endemic tularemia.