Determination of properties of individual liposomes by capillary electrophoresis with postcolumn laser-induced fluorescence detection
Determination of properties of individual liposomes by capillary electrophoresis with postcolumn laser-induced fluorescence detection
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DOI：
10.1021/ac0010330
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发表时间：
2001-04-15
影响因子：
7.4
通讯作者：
Arriaga, EA
中科院分区：
文献类型：
--
作者：
Duffy, CF;Gafoor, S;Arriaga, EA
通过毛细管电泳柱后激光诱导荧光检测的单个脂质体测量促进了脂质体性质分布的测定，二维图，以及脂质体制剂的改进表征。这一进步在脂质体分析是可行的，通过使用高灵敏度柱后激光诱导荧光检测器有线毫秒响应。对于含有荧光素的每个单独的脂质体，测定峰高和迁移时间。从这些测量的个人截留体积和电泳迁移率进行了测定。这些特性的分布分析有助于比较各种脂质体稀释液，并表明该方法具有重现性，且不受混悬液中脂质体密度（10（7）-10（9）脂质体/ml）的影响。此外，脂质体的包封体积为0.3至13 fL，表观半径为370 nm至1.8 μ m。二维图的减少流动性与kappaR（德拜参数x脂质体半径）显示，从干膜的磷脂重悬的脂质体是异质性方面的单个脂质体的表面电荷密度。所描述的方法有可能成为一个新的工具，商业脂质体制剂的表征和理论研究。
Individual liposome measurements by capillary electrophoresis with postcolumn laser-induced fluorescence detection facilitated the determination of liposome property distributions, two-dimensional plots, and an improved characterization of a liposomal preparation. This advancement in liposome analysis was feasible by using a high-sensitivity postcolumn laser-induced fluorescence detector wired for millisecond response. For each individual liposome containing fluorescein, peak height and migration time were determined. From these measurements the individual entrapped volumes and electrophoretic mobilities were determined. Distribution analysis of these properties facilitated comparison of various liposome dilutions and indicated that the method is reproducible and unaffected by the density of liposomes (10(7)-10(9) liposomes/ml) in the suspension. Furthermore, liposomes showed entrapped volumes that vary from 0.3 to 13 fL with apparent radius varying from 370 nm to 1.8 mum. Two-dimensional plots of reduced mobility versus kappaR (Debye parameter x liposome radius) revealed that the liposomes resuspended from a dried film of phospholipids are heterogeneous in regard to the surface charge density of individual liposomes. The described method has the potential of becoming a new tool for characterization of commercial liposomal preparations and theoretical studies.