Multifunctional GQDs-Concanavalin A@Fe3O4 nanocomposites for cancer cells detection and targeted drug delivery
Multifunctional GQDs-Concanavalin A@Fe3O4 nanocomposites for cancer cells detection and targeted drug delivery
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DOI：
10.1016/j.aca.2018.04.029
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发表时间：
2018-10-16
影响因子：
6.2
通讯作者：
Doong, Ruey-an
中科院分区：
文献类型：
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作者：
Chowdhury, Ankan Dutta;Ganganboina, Akhilesh Babu;Doong, Ruey-an
含有作为传感元件的固有特性的多功能纳米复合材料以及靶向纳米复合物在各种治疗应用中是高度优选的。在这项工作中，开发了石墨烯量子点（GQD）和 Fe3O4 纳米复合材料，与凝集素蛋白伴刀豆球蛋白 A 结合形成 GQD-ConA@ Fe3O4 纳米复合材料，用于检测癌细胞和向 HeLa 细胞释放药物。沉积在Pt电极上的GQD-ConA@ Fe3O4纳米复合材料可以检测正常内皮细胞上的癌性HeLa细胞，动态线性范围为5 x 10(2)至1 x 10(5) cells mL(-1)，检测限为273 cell mL(-1)。 GQD-ConA@ Fe3O4 还可以作为纳米载体用于负载和递送阿霉素 (Dox)。体外细胞图像显示，由于纳米载体中掺入了 Fe3O4，HeLa 细胞中的 Dox 浓度在外部磁场存在下提高了一倍以上。细胞毒性测定表明，癌性HeLa细胞对Dox的敏感性比正常细胞高13%，证实了ConA在纳米载体中的选择性作用。结果清楚地表明，GQD-ConA@ Fe3O4 纳米复合材料是一种有前途的材料，可用于癌细胞检测和向 HeLa 细胞定向释放 Dox，可作为新型癌细胞诊断和治疗应用的多功能平台。 (C) 2018 Elsevier B.V. 保留所有权利。
Multifunctional nanocomposites containing intrinsic property for serving as the sensing elements as well as targeted nanoconjugates are highly preferred in various therapeutic applications. In this work, nanocomposites of graphene quantum dots (GQDs) and Fe3O4 with conjugation of lectin protein, concanavalin A, to form GQD-ConA@ Fe3O4 nanocomposites are developed for both detection of cancer cell and release of drugs to HeLa cells. The GQD-ConA@ Fe3O4 nanocomposites deposited on Pt electrode can detect cancerous HeLa cells over normal endothelial cells with a dynamic linear range of 5 x 10(2) to 1 x 10(5) cells mL(-1) with a detection limit of 273 cell mL(-1). The GQD-ConA@ Fe3O4 also can serve as nanocarriers for loading and delivering doxorubicin (Dox). The in vitro cell images show that the Dox concentration in HeLa cells is enhanced more than double in the presence of external magnetic field due to the incorporation of Fe3O4 in the nanocarrier. The cytotoxicity assay indicates that the susceptibility of cancerous HeLa cells to Dox is 13% higher than that of normal cells, confirming the selective role of ConA in nanocarriers. Results clearly indicate the GQD-ConA@ Fe3O4 nanocomposites as a promising material for cancer cell detection and targeted Dox release toward HeLa cells which can serve as the multifunctional platform for novel cancer cell diagnostic and therapeutic applications. (C) 2018 Elsevier B.V. All rights reserved.