Efficient postacquisition synchronization of 4-D nongated cardiac images obtained from optical coherence tomography: application to 4-D reconstruction of the chick embryonic heart
Efficient postacquisition synchronization of 4-D nongated cardiac images obtained from optical coherence tomography: application to 4-D reconstruction of the chick embryonic heart
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DOI：
10.1117/1.3184462
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发表时间：
2009-07-01
影响因子：
3.5
通讯作者：
Rugonyi, Sandra
中科院分区：
文献类型：
--
作者：
Liu, Aiping;Wang, Ruikang;Rugonyi, Sandra
胚胎心脏的四维（4-D）成像允许在发育过程中研究体内心脏的形态和功能。然而，4-D成像的胚胎心脏使用当前的技术，包括光学相干断层扫描（OCT），是有限的图像采集速率。在这里，我们提出了一个nongated 4-D成像策略结合一个有效的采集后同步程序，规避了采集速率的限制。4-D成像策略在B模式下采集心脏沿着几个不同位置的时间序列图像，呈现异相图像序列。然后，我们的同步过程使用的局部结构的相似性，以找到相邻的图像序列之间的相移，采用M模式图像（从所获得的B模式图像提取），以实现计算效率。此外，我们的程序纠正了相移，通过考虑胚胎心脏壁的类心房收缩所引入的相位滞后。我们应用4-D成像策略和同步程序来重建鸡胚的心脏流出道（OFT），在发育的早期阶段（Hamburger-Hamilton阶段18）用OCT成像。我们表明，所提出的同步程序实现效率，而不牺牲准确性，重建的4-D图像正确捕获的OFT室壁运动的动态。(C)2009年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。[DOI 10.1117/1.3184462]
Four-dimensional (4-D) imaging of the embryonic heart allows study of cardiac morphology and function in vivo during development. However, 4-D imaging of the embryonic heart using current techniques, including optical coherence tomography (OCT), is limited by the rate of image acquisition. Here, we present a nongated 4-D imaging strategy combined with an efficient postacquisition synchronization procedure that circumvents limitations on acquisition rate. The 4-D imaging strategy acquires a time series of images in B mode at several different locations along the heart, rendering out-of-phase image sequences. Then, our synchronization procedure uses similarity of local structures to find the phase shift between neighboring image sequences, employing M-mode images (extracted from the acquired B-mode images) to achieve computational efficiency. Furthermore, our procedure corrects the phase shifts by considering the phase lags introduced by peristaltic-like contractions of the embryonic heart wall. We applied the 4-D imaging strategy and synchronization procedure to reconstruct the cardiac outflow tract (OFT) of a chick embryo, imaged with OCT at early stages of development (Hamburger-Hamilton stage 18). We showed that the proposed synchronization procedure achieves efficiency without sacrificing accuracy and that the reconstructed 4-D images properly captured the dynamics of the OFT wall motion. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3184462]