Exogenous collagen cross-linking recovers tendon functional integrity in an experimental model of partial tear
Exogenous collagen cross-linking recovers tendon functional integrity in an experimental model of partial tear
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DOI：
10.1002/jor.22014
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发表时间：
2012-06-01
影响因子：
2.8
通讯作者：
Snedeker, Jess G.
中科院分区：
文献类型：
--
作者：
Fessel, Gion;Wernli, Jeremy;Snedeker, Jess G.
我们研究了外源性胶原交联可以增加肌腱完整区域以减轻部分撕裂的机械传播的假设。我们首先筛选了低毒性的胶原交联剂京尼平，甲基乙二醛和紫外线（UV）光的能力，以增加肌腱刚度和失败的负荷在大鼠尾腱束（RTTF）。然后，我们研究了在承重马浅趾屈肌腱（SDFT）的交联效果。数据表明，所有三种交联剂都增强了RTTF的机械性能，但降低了固有粘弹性。与在纤维束中观察到的效果相反，甲基乙二醛处理SDFT会在精神上影响肌腱的机械完整性，并且在UV的情况下不会改变肌腱力学。如在RTTF实验中，SDFT的京尼平交联导致刚度增加、破坏载荷更高和粘弹性降低。基于这一结果，我们评估了京尼平在阻止肌腱撕裂在循环载荷下扩展至失效的功效。继发于中间物质活检穿孔的京尼平交联显著降低了组织应变，增加了弹性模量并增加了对疲劳失效的抵抗力。我们的结论是京尼平交联受伤的肌腱有可能阻止肌腱撕裂的进展，现在应该研究的影响，在活肌腱的基质重塑的治疗。(c)2011骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 30：973981，2012
We investigated the hypothesis that exogenous collagen cross-linking can augment intact regions of tendon to mitigate mechanical propagation of partial tears. We first screened the low toxicity collagen cross-linkers genipin, methylglyoxal and ultra-violet (UV) light for their ability to augment tendon stiffness and failure load in rat tail tendon fascicles (RTTF). We then investigated cross-linking effects in load bearing equine superficial digital flexor tendons (SDFT). Data indicated that all three cross-linking agents augmented RTTF mechanical properties but reduced native viscoelasticity. In contrast to effects observed in fascicles, methylglyoxal treatment of SDFT detrimentally affected tendon mechanical integrity, and in the case of UV did not alter tendon mechanics. As in the RTTF experiments, genipin cross-linking of SDFT resulted in increased stiffness, higher failure loads and reduced viscoelasticity. Based on this result we assessed the efficacy of genipin in arresting tendon tear propagation in cyclic loading to failure. Genipin cross-linking secondary to a mid-substance biopsy-punch significantly reduced tissue strains, increased elastic modulus and increased resistance to fatigue failure. We conclude that genipin cross-linking of injured tendons holds potential for arresting tendon tear progression, and that implications of the treatment on matrix remodeling in living tendons should now be investigated. (c) 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:973981, 2012