Occurrence of Tetrodotoxin in a Trumpet Shell, “Boshubora” Charonia sauliae
Occurrence of Tetrodotoxin in a Trumpet Shell, “Boshubora” Charonia sauliae
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喇叭壳“Boshubora”Charonia sauliae 中存在河豚毒素
DOI：
10.2331/suisan.47.935
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发表时间：
1981
影响因子：
0.4
通讯作者：
K. Hida
中科院分区：
文献类型：
--
作者：
H. Narita;T. Noguchi;J. Maruyama;Y. Ueda;K. Hashimoto;Y. Watanabe;K. Hida
1979年12月，由于误食了在静冈县Miho捕获的一种名为“boshubora”Charonia sauliae的喇叭壳，导致了一种麻痹性中毒。毒性研究表明，在那里收集的大多数boshubora标本在消化腺处都有毒性。采用活性炭处理，Amberlite IRC-50和CM-Sephadex C-25层析，最后结晶的方法从消化腺中纯化boshubora毒素（以下简称BST）。重结晶毒素毒性为3700 μ /mg。BST的薄层色谱和电泳结果与河豚毒素（TTX）的薄层色谱和电泳结果一致，可供参考。Furthermoree。此外，两种毒素的红外光谱和红外光谱、c9碱基三甲基硅基衍生物的质谱和剂量-死亡时间曲线吻合良好。根据这些结果，BST被鉴定为TTX。
A paralytic poisoning occurred in Deccember, 1979, due to ingestion of a trumpet shell, “boshubora” Charonia sauliae which was caught off Miho, Shizuoka Prefecture. The toxicity study showed that most of the boshubora specimens collected around there were toxic at the digestive gland. The boshubora toxin (abbreviated as BST below) was purified from the digestive gland by a method which consisted of the treatment with activated charcoal, chromatography on Amberlite IRC-50 and CM-Sephadex C-25, and finally crystallixation. The recrystallixed toxin shwed a toxicity of 3, 700 MU/mg. Thin-layer chromatographic and electrophoretic results for BST were the same as those for tetrodotoxin (TTX) analyzed for reference. Furthermoree. Furthermore, both toxins coincided well in their IR and NM Rspectra, the mass spectra of the C9-base-trimethylsilyl derivative, and the dose-death time curves. Based on these results, BST was identified as TTX.