Low dopamine transporter mRNA levels in midbrain regions containing calbindin.
Low dopamine transporter mRNA levels in midbrain regions containing calbindin.
复制标题
含有钙结合蛋白的中脑区域多巴胺转运蛋白 mRNA 水平低。
DOI：
10.1097/00001756-199408150-00025
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发表时间：
1994
期刊：
影响因子：
1.7
通讯作者：
German,DC
中科院分区：
文献类型：
--
作者：
Sanghera,MK;Manaye,KF;Liang,CL;Lacopino,AM;Bannon,MJ;German,DC
多巴胺转运蛋白（DAT）是MPTP的神经毒性代谢物进入中脑多巴胺能（DA）神经元的位点。然而，并不是所有的中脑DA神经元变性后MPTP处理。与缺乏CALB的DA神经元相比，含有钙结合蛋白-D 2*（CALB）的中脑DA神经元相对不易受MPTP毒性的影响。使用原位杂交和免疫细胞化学染色技术在大鼠和小鼠，我们现在报告，有多达10倍少的DAT mRNA的DA神经元含有CALB的区域相比，DA神经元缺乏CALB的区域。这些数据表明，特定的中脑DA神经元是无懈可击的MPTP毒性，不仅因为它们含有CALB，但也因为它们具有相对较低的DAT活性。
THE dopamine transporter (DAT) is the site at which the neurotoxic metabolite of MPTP gains access to midbrain dopaminergic (DA) neurons. However, not all midbrain DA neurons degenerate following MPTP treatment. The midbrain DA neurons that contain the calcium-binding protein, calbindin-D 2***(CALB), are relatively invulnerable to MPTP toxicity, compared with DA neurons that lack CALB. Using in situ hybridization and immunocy-tochemical staining techniques in the rat and mouse, we now report that there is as much as 10 fold less DAT mRNA in regions where DA neurons contain CALB compared with regions where DA neurons lack CALB. These data suggest that specific midbrain DA neurons are invulnerable to MPTP toxicity not only because they contain CALB, but also because they have relatively low DAT activity.