Sequence requirements for synthetic peptide-mediated translocation to the nucleus
Sequence requirements for synthetic peptide-mediated translocation to the nucleus
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合成肽介导的易位至细胞核的序列要求
DOI：
10.1128/mcb.9.6.2487-2492.1989
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发表时间：
1989
影响因子：
5.3
通讯作者：
Experiinenital Staitioni
中科院分区：
文献类型：
--
作者：
D. Chelsky;Rebecca Ralph;G. Jonak;Cenitral;Experiinenital Staitioni
测定了18个合成肽在HeLa细胞胞浆内显微注射后将载体蛋白靶向细胞核的能力。选择用于合成的序列中有8个是基于通过基因融合和缺失实验确定的已公布的核靶向区。这些序列中有六个被合成的多肽模拟并连接到载体蛋白时是有效的。另一个多肽是基于非洲爪哇核蛋白lamin L1的一个区域，在这个区域中，核靶向区域以前从未被研究过。这种多肽还能够将载体蛋白靶向细胞核。另外八个与已知靶向信号相似的多肽几乎没有或几乎没有核靶向能力。能够将载体蛋白定位到细胞核的多肽在注入细胞质的45分钟内就能做到这一点。两个在45min后几乎没有明显核靶向能力的多肽也被检测了更长的时间。胞浆注射后45min~4h，核内积聚未见增加。对有效的核靶向序列与那些没有发现多肽介导的核运输可能的共识序列的序列进行比较。
The abilities of 18 synthetic peptides to target a carrier protein to the nucleus following microinjection into the cytoplasm of HeLa cells were determined. Eight of the sequences chosen for synthesis were based on published nuclear targeting regions as determined by gene fusion and deletion experiments. Six of these sequences were found to be effective when mimicked by a synthetic peptide and conjugated to a carrier protein. One additional peptide was based on a region of lamin L1, a nuclear protein from Xenopus laevis, in which the nuclear targeting region had not been previously investigated. This peptide was also able to target a carrier protein to the nucleus. Eight other peptides which resemble the known targeting signals had little or no nuclear targeting ability. Peptides which were able to target a carrier protein to the nucleus did so within 45 min of injection into the cytoplasm. Two peptides with little or no apparent nuclear targeting ability after 45 min were examined for longer times as well. No increase in nuclear accumulation was observed between 45 min and 4 h after cytoplasmic injection. Comparison of the sequences which were effective at nuclear targeting with those that were not revealed a possible consensus sequence for peptide-mediated nuclear transport.
细胞核：结构、功能和动力学。
影响因子：
16.6
作者：
Newport,JW;Forbes,DJ
通讯作者：
Forbes,DJ
碱性和非碱性氨基酸取代对猿病毒40 T抗原合成肽核转运信号诱导转运的影响。
影响因子：
5.3
作者：
Lanford,RE;White,RG;Dunham,RG;Kanda,P
通讯作者：
Kanda,P