Analysis of omeprazole, midazolam and hydroxy-metabolites in plasma using liquid chromatography coupled to tandem mass spectrometry
Analysis of omeprazole, midazolam and hydroxy-metabolites in plasma using liquid chromatography coupled to tandem mass spectrometry
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DOI：
10.1016/j.jchromb.2005.07.001
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发表时间：
2005-09-25
影响因子：
3
通讯作者：
Haas, CE
中科院分区：
文献类型：
--
作者：
Frerichs, VA;Zaranek, C;Haas, CE
开发并验证了一种方法，该方法采用高效液相色谱串联质谱法定量测定250 μ L人血浆样品中的咪达唑仑、α羟基咪达唑仑、奥美拉唑和羟基奥美拉唑。该方法经验证的每日工作范围为0.400-100 ng/mL，检测限为2 - 15 pg/mL。在4种对照浓度下，所有分析物的试验间变异小于15%，并且样品在分析条件下可稳定保存3次冻融循环，在制备后分析基质中可稳定保存24 h。该方法用于分析样品，以支持探索细胞色素P-450酶系统活性的临床研究。(c)2005 Elsevier B. V.保留所有权利。
A method has been developed and validated for the quantitation of midazolam, alphahydroxy-midazolam, omeprazole, and hydroxyomeprazole from one 250 mu L sample of human plasma using high performance liquid chromatography coupled to tandem mass spectrometry. The method was validated for a daily working range of 0.400-100 ng/mL, with limits of detection between 2 and 15 pg/mL. The inter-assay variation was less than 15% for all analytes at four control concentrations and the samples were stable for three freeze-thaw cycles under the analysis conditions and 24 h in the post-preparative analysis matrix. This method was used to analyze samples in support of clinical studies probing the activity of the cytochrome P-450 enzyme system. (c) 2005 Elsevier B.V. All rights reserved.