Assaying genome-wide recombination and centromere functions with Arabidopsis tetrads
Assaying genome-wide recombination and centromere functions with Arabidopsis tetrads
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DOI：
10.1073/pnas.95.1.247
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发表时间：
1998-01-06
影响因子：
11.1
通讯作者：
Preuss, D
中科院分区：
文献类型：
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作者：
Copenhaver, GP;Browne, WE;Preuss, D
在减数分裂过程中，交叉事件产生新的等位基因组合，但这些基因交换在单个细胞中的丰度以前没有在基因组水平上测量。为了进行全基因组重组分析，我们监测了拟南芥减数分裂四分体中遗传标记的分类。通过确定在个别减数分裂细胞中的交换的数量和分布，我们证明了（i）在每次减数分裂中交换数量的令人惊讶的精确调节，（ii）沿着携带核糖体DNA阵列的染色体的重组沿着减少，以及（iii）重组频率和染色体大小之间的反比关系。这种四分体分析的使用也实现了所有五个拟南芥着丝粒的精确定位，定位在高等真核生物的完整染色体中的着丝粒功能。
During meiosis, crossover events generate new allelic combinations, yet the abundance of these genetic exchanges in individual cells has not been measured previously on a genomic level. To perform a genome-wide analysis of recombination, we monitored the assortment of genetic markers in meiotic tetrads from Arabidopsis. By determining the number and distribution of crossovers in individual meiotic cells, we demonstrated (i) surprisingly precise regulation of crossover number in each meiosis, (ii) considerably reduced recombination along chromosomes carrying ribosomal DNA arrays, and (iii) an inversely proportional relationship between recombination frequencies and chromosome size. This use of tetrad analysis also achieved precise mapping of all five Arabidopsis centromeres, localizing centromere functions in the intact chromosomes of a higher eukaryote.