Organization of connectivity of the rat presubiculum: I. Efferent projections to the medial entorhinal cortex
Organization of connectivity of the rat presubiculum: I. Efferent projections to the medial entorhinal cortex
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DOI：
10.1002/cne.20093
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发表时间：
2004-06-07
影响因子：
2.5
通讯作者：
Ishizuka, N
中科院分区：
文献类型：
--
作者：
Honda, Y;Ishizuka, N
用菜豆白细胞凝集素顺行标记和辣根过氧化物酶结合麦胚凝集素逆行标记，研究了大鼠前下托到内嗅区的层状和地形投射的组织结构。我们发现前下托-内嗅投射的模式在前下托的浅层和深层之间是不同的。前下托的浅层（第二和第三层）产生了双边的预测，第一至第六层的内侧内嗅区（MEA）。许多终端分布在第三层，较少的在第二层和第一层的深部，和更少的终端在深层（第五层和第六层）的MEA。与此相反，深层（层V和VI）的前下托引起同侧的预测内嗅区。MEA的V、VI层和I层的最浅部有较多轴突终末分布，而II、III层轴突终末分布较少。此外，第I层的分支延伸到外侧内嗅区（莱亚）。利用海马旁皮质的二维展开图，我们阐明了前下托-内嗅投射的独特地形关系：1）前下托的隔颞轴或纵轴与MEA/莱亚边界上的轴相对应，其中隔前下托向鼻裂投射，颞前下托向鼻裂投射。2)前下托的近远轴对应于从MEA/莱亚边界到MEA/旁下托边界的轴，该轴实际上垂直于MEA/莱亚边界，其中前下托的近端部分（靠近下托）投影到MEA/莱亚边界附近的区域。(C)2004 Wiley-Liss，Inc.
The organization of the laminar and topographical projections from the presubiculum to the entorhinal area was studied in the rat by anterograde labeling with Phaseolus vulgaris leucoagglutinin and retrograde labeling with horseradish peroxidase conjugated to wheat germ agglutinin. We found that the pattern of presubiculo-entorhinal projections differs between the superficial and deep layers of the presubiculum. The superficial layers (layers II and III) of the presubiculum gave rise to bilateral projections to layers I-VI of the medial entorhinal area (MEA). Many terminals were distributed in layer III, fewer in layer II and the deep portion of layer I, and many fewer terminals in the deep layers (layers V and VI) of MEA. In contrast, the deep layers (layers V and VI) of the presubiculum gave rise to ipsilateral projections to the entorhinal area. Many axon terminals were distributed in layers V and VI of MEA and the most superficial portion of layer I of MEA, but very few in layers II and III. In addition, the ramifications in layer I extended to the lateral entorhinal area (LEA). Using two-dimensional unfolded maps of parahippocampal cortices, we elucidated the distinct topographical relationship in the presubiculo-entorhinal projection: 1) The septotemporal or longitudinal axis of the presubiculum corresponded to the axis on the MEA/LEA boundary, where the septal presubiculum projected toward the rhinal fissure and the temporal presubiculum projected away from the fissure. 2) The proximodistal axis of the presubiculum corresponded to the axis from the MEA/LEA boundary to the MEA/parasubiculum boundary that was virtually perpendicular to the MEA/LEA boundary, where the proximal portion of the presubiculum (close to the subiculum) projected to the region near the MEA/LEA boundary. (C) 2004 Wiley-Liss, Inc.