Transcriptome analysis of Bacillus coagulans P38, an efficient producer of L-lactic acid from cellulosic hydrolysate, in response to 2-furfural stress
Transcriptome analysis of Bacillus coagulans P38, an efficient producer of L-lactic acid from cellulosic hydrolysate, in response to 2-furfural stress
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DOI：
10.1007/s13213-015-1179-x
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发表时间：
2016-06
影响因子：
3
通讯作者：
Xiuwen Wang;J. Qin;Qingqing Zhu;Beibei Zhu;Xiaohua Zhang;Qingshou Yao
中科院分区：
文献类型：
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作者：
Xiuwen Wang;J. Qin;Qingqing Zhu;Beibei Zhu;Xiaohua Zhang;Qingshou Yao
通过比较转录组分析，研究了2-糠醛胁迫和无2-糠醛胁迫条件下乳酸产杆菌凝固芽孢杆菌（bacillus coagulansP38）的表达谱。在7 g l−12-糠醛胁迫下，b。凝血酶38表达降低，740个基因表达上调，540个基因表达下调。KEGG通路富集分析显示，2-糠醛胁迫对9条通路有显著影响（P< 0.01）。糖酵解/糖异生的下调和三羧酸循环循环的上调表明，2-糠醛胁迫对l -乳酸发酵有负面影响。基因本体鉴定显示，在2-糠醛胁迫下，5个转运蛋白和1个转录调节因子上调了6倍以上。2-糠醛胁迫诱导了7个醇脱氢酶基因和8个短链脱氢酶/还原酶基因的表达，这些基因可能参与了糠醛耐受性的调节。我们的发现为b的反应提供了新的见解。2-糠醛应激凝固剂。此外，我们的RNA-seq数据集构成了一个强大的数据库，可用于研究2-糠醛胁迫诱导的遗传功能。
A comparative transcriptome analysis was conducted to study the expression profiles of the lactic acid-producing strainBacillus coagulansP38 cultivated in the presence and absence of 2-furfural stress. In response to 7 g l−12-furfural stress, the cell growth rate ofB. coagulansP38 was reduced, and a total of 740 genes were upregulated and 540 genes downregulated. KEGG pathway enrichment analysis revealed that nine pathways were significantly affected by 2-furfural stress (P< 0.01). The downregulation of glycolysis/gluconeogenesis and upregulation of the tricarboxylic acid cycle cycle revealed thatL-lactic acid fermentation was negatively affected by 2-furfural stress. Gene Ontology assignments showed that five transporters and one transcriptional regulator were upregulated by more than sixfold under 2-furfural stress. The expression of seven alcohol dehydrogenase genes and eight short-chain dehydrogenase/reductase genes, all of which may be involved in the mediation of furfural tolerance, were induced under 2-furfural stress. Our findings offer new insights into the response ofB. coagulansto 2-furfural stress. In addition, our RNA-seq dataset constitutes a robust database that can be used to investigate the genetic functions induced by 2-furfural stress.