Vomiting and wasting disease associated with hemagglutinating encephalomyelitis viruses infection in piglets in Jilin, China.
Vomiting and wasting disease associated with hemagglutinating encephalomyelitis viruses infection in piglets in Jilin, China.
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DOI：
10.1186/1743-422x-8-130
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发表时间：
2011-03-21
期刊：
影响因子：
4.8
通讯作者：
Gao F
中科院分区：
文献类型：
--
作者：
Gao W;Zhao K;Zhao C;Du C;Ren W;Song D;Lu H;Chen K;Li Z;Lan Y;Xie S;He W;Gao F
从吉林省10头临床表现为呕吐、腹泻和运动障碍的仔猪脑组织中分离到1株冠状病毒。采用多重PCR、负染电镜和血凝素酯酶（Hemagglutininin-esterase，HE）基因序列分析等方法，对PHEV-JLsp 09株进行了抗原性和基因组学研究。这些仔猪被诊断患有猪血凝性脑脊髓炎病毒（PHEV）。对仔猪进行尸检。主要病理改变为脑膜充血、脑膜出血和皮质出血。在其他器官中也观察到轻微变化。组织学改变包括大脑皮质中的卫星化和神经元吞噬。用分离的病毒感染小鼠。它们的组织病理学变化与在小猪中观察到的症状相似，表现出非化脓性脑炎的典型变化。因此，猪血凝性脑脊髓炎病毒主要对神经系统造成损害，但也影响其他器官。在中国吉林省四平地区发现的该病毒株（分离株PHEV-JLsp 09）在进化上最接近HEV-67 N株（北美株），表明该病毒株是由来自北美的PHEV进化而来的。
One coronavirus strain was isolated from brain tissues of ten piglets with evident clinical manifestations of vomiting, diarrhea and dyskinesia in Jilin province in China. Antigenic and genomic characterizations of the virus (isolate PHEV-JLsp09) were based on multiplex PCR and negative staining electron microscopy and sequence analysis of the Hemagglutinin-esterase (HE) gene. These piglets were diagnosed with Porcine hemagglutinating encephalomyelitis virus (PHEV). Necropsy was performed on the piglets. Major pathological changes included meningeal hyperemia, meningeal hemorrhage and cortical hemorrhage. Minor changes were also observed in other organs. Histopathological changes included satellitosis and neuronophagia in the cerebral cortex. Mice were infected with the isolated virus. Their histopathological changes were similar to those symptoms observed in the piglets, exhibiting typical changes for non-suppurative encephalitis. Thus, Porcine hemagglutinating encephalomyelitis virus mainly causes damage to the nervous system but also impacts other organs. This viral strain (isolate PHEV-JLsp09) found in the Siping area of Jilin Province in China is evolutionally closest to the HEV-67N stain (North American strain), indicating that this viral strain evolved from the PHEV from North America.