Environmental modulation of exopolysaccharide production in the cyanobacterium Synechocystis 6803.
Environmental modulation of exopolysaccharide production in the cyanobacterium Synechocystis 6803.
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DOI：
10.1007/s00253-023-12697-9
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发表时间：
2023-10
影响因子：
5
通讯作者：
Amtmann, Anna
中科院分区：
文献类型：
--
作者：
Madsen, Mary Ann;Semerdzhiev, Stefan;Twigg, Jordan D.;Moss, Claire;Bavington, Charles D.;Amtmann, Anna
关键词：
微生物产生多种组成和结构的胞外聚合物（EPS，也称为胞外多糖）。这些生物聚合物的生物化学和生物物理特性使得广泛的工业应用成为可能。蓝细菌的EPS是特别通用的，因为它们包含了更多数量和种类的构建模块，并采用比其他生物的EPS更复杂的结构。然而，蓝藻中EPS生物合成途径的遗传组成和调控知之甚少。在这里，我们测量了改变培养基对集胞藻PCC 6803释放的EPS的滴度和组成的影响，并将此信息与转录组学数据相结合。在所有条件下，单个细胞的每日EPS生产率在早期生长阶段最高，但由于积累，从培养物中获得的EPS总量在后期生长阶段最高。降低培养基中的镁浓度提高了每个细胞的生产力，但产生的EPS具有较低的总糖含量。单个单糖的水平与特定培养基组分相关，例如木糖与硫、葡萄糖和N-乙酰基-半乳糖胺与NaCl。与RNA测序数据的比较表明，Wzy依赖的生物合成途径和富含木糖的EPS的保护作用。这种多层次的分析提供了一个把手，将单个基因与复杂生物聚合物的动态调节联系起来。·集胞藻胞外多糖的量和组成取决于培养条件·生产速率和糖含量可分别由Mg和S调节·Wzy依赖的生物合成途径和对富含木糖的EPS的保护作用在线版本包含补充材料，可在10. 1007/s 00253 -023-12697-9获得。
Microorganisms produce extracellular polymeric substances (EPS, also known as exopolysaccharides) of diverse composition and structure. The biochemical and biophysical properties of these biopolymers enable a wide range of industrial applications. EPS from cyanobacteria are particularly versatile as they incorporate a larger number and variety of building blocks and adopt more complex structures than EPS from other organisms. However, the genetic makeup and regulation of EPS biosynthetic pathways in cyanobacteria are poorly understood. Here, we measured the effect of changing culture media on titre and composition of EPS released by Synechocystis sp. PCC 6803, and we integrated this information with transcriptomic data. Across all conditions, daily EPS productivity of individual cells was highest in the early growth phase, but the total amount of EPS obtained from the cultures was highest in the later growth phases due to accumulation. Lowering the magnesium concentration in the media enhanced per-cell productivity but the produced EPS had a lower total sugar content. Levels of individual monosaccharides correlated with specific culture media components, e.g. xylose with sulfur, glucose and N-acetyl-galactosamine with NaCl. Comparison with RNA sequencing data suggests a Wzy-dependent biosynthetic pathway and a protective role for xylose-rich EPS. This multi-level analysis offers a handle to link individual genes to the dynamic modulation of a complex biopolymer. • Synechocystis exopolysaccharide amount and composition depends on culture condition • Production rate and sugar content can be modulated by Mg and S respectively • Wzy-dependent biosynthetic pathway and protective role proposed for xylose-rich EPS The online version contains supplementary material available at 10.1007/s00253-023-12697-9.
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影响因子：
3.7
作者：
Jittawuttipoka T;Planchon M;Spalla O;Benzerara K;Guyot F;Cassier-Chauvat C;Chauvat F
通讯作者：
Chauvat F
来自海洋微藻的硫化多糖的生物活性和应用。
影响因子：
5.4
作者：
Raposo MF;de Morais RM;Bernardo de Morais AM
通讯作者：
Bernardo de Morais AM
影响因子：
6.3
作者：
Chen L;Wu L;Wang J;Zhang W
通讯作者：
Zhang W
影响因子：
5.7
作者：
Madsen MA;Hamilton G;Herzyk P;Amtmann A
通讯作者：
Amtmann A
影响因子：
5.1
作者：
Flores, Carlos;Santos, Marina;Tamagnini, Paula
通讯作者：
Tamagnini, Paula