Restricted access magnetic imprinted microspheres for directly selective extraction of tetracycline veterinary drugs from complex samples
Restricted access magnetic imprinted microspheres for directly selective extraction of tetracycline veterinary drugs from complex samples
复制标题
限制访问磁性印迹微球用于从复杂样品中直接选择性提取四环素兽药
DOI：
10.1016/j.chroma.2019.460684
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发表时间：
--
影响因子：
4.1
通讯作者：
Bolin Gong
中科院分区：
文献类型：
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作者：
Yanqiang Zhou;Bolin Gong
制备了磁性分子印迹聚合物(RAM-MMIPs)作为四环素类药物的磁性固相萃取(M-SPE)材料。RAM-MMIPs不仅能特异地吸附样品中的目标分子，而且能有效消除蛋白质大分子的干扰。蛋白质排斥率为99.4%。此外，RAM-MMIP具有均匀的印迹和亲水层(600 Nm)、快速结合动力学(35min)、高选择性和较大的吸附容量。将M-SPE与HPLC/UV联用，从未经处理的牛奶和鸡蛋样品中提取天花粉蛋白，并对影响M-SPE效率的几个主要因素进行了优化。在优化的条件下，该方法获得了良好的线性关系(R2>；0.9989)、较低的检出限和较高的回收率。牛奶样品的检出限为1.03~1.31μg L−1，回收率为86.7%~98.6%，相对标准偏差为1.4%~5.7%。鸡蛋样品的检出限为2.21~2.67μg L−1，回收率为84.2%~96.5%，相对标准偏差为1.7%~5.9%。因此，这项工作为直接从含有蛋白质的复杂样品中选择性地提取和检测目标分子提供了一种改进的策略。
A novel restricted access media-magnetic molecularly imprinted polymers (RAM-MMIPs) was prepared as magnetic-solid phase extraction (M-SPE) material for tetracyclines (TCs). The RAM-MMIPs can not only specifically adsorb target molecules in samples, but also effectively eliminate the interference of protein macromolecules. The protein exclusion rate is 99.4%. Besides, RAM-MMIPs have a uniform imprinted and hydrophilic layer (600 nm), rapid binding kinetic (35 min), high selectivity and larger adsorption capacity. The M-SPE was coupled with HPLC/UV to extract TCs from untreated milk and egg samples, and sev- eral major factors affecting M-SPE efficiency were optimized. Under optimized conditions, the developed method achieved good linearity (R 2 > 0.9989), lower limits of detection (LOD) and higher recoveries of TCs. For milk samples, the LOD is 1.03–1.31 μg L −1 and the recovery is 86.7% to 98.6% with relative standard deviation (RSD) of 1.4–5.7%. For the egg samples, the LOD, recovery and RSD are 2.21–2.67 μg L −1 , 84.2–96.5% and 1.7–5.9%, respectively. Consequently, this work provides an improved strategy for the selective extraction and detection of target molecules directly from complex samples with proteins.