Gut hormones in stimulation of gastroduodenal alkaline secretion in conscious dogs.
Gut hormones in stimulation of gastroduodenal alkaline secretion in conscious dogs.
复制标题
肠道激素刺激清醒狗胃十二指肠碱性分泌。
DOI：
10.1152/ajpgi.1985.248.6.g687
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
J. Laskiewicz
中科院分区：
文献类型：
--
作者：
S. Konturek;J. Bilski;J. Tasler;J. Laskiewicz
在清醒的犬中，在基础条件下和胃内滴注盐酸溶液、喂肉或静脉输注各种肠道激素后，测量胃底和胃窦囊以及近端和远端十二指肠袢的碱性分泌。在对照试验中，禁食狗的十二指肠粘膜的HCO-3输出是胃粘膜的几倍。10 mM HCl注入胃导致HCO-3从胃囊和近端十二指肠袢分泌的显著增加，这是伴随着血浆促胰液素，胆囊收缩素（CCK）和胰多肽（PP）水平的显着增加。肉食刺激了近端十二指肠HCO-3的分泌，并伴随着血浆胃泌素、促胰液素、CCK、抑胃肽和PP的显著升高。在外源性激素中，最有效的HCO-3分泌刺激剂是PP，其在使血浆PP升高至餐后水平的剂量（125-500 pmol X kg-1 X h-1）下引起胃和十二指肠粘膜的HCO-3输出的显著增加。生理剂量（21-85 pmol.kg-1.h-1）的CCK也刺激胃囊和近端十二指肠袢的HCO-3分泌。神经降压素刺激HCO-3分泌从胃袋和十二指肠袢。与此相反，胃泌素或促胰液素没有显着影响HCO-3从胃十二指肠粘膜分泌。这项研究提供的证据表明，一些肠道激素，特别是PP，CCK和神经降压素，可能参与胃十二指肠碱性分泌的生理刺激。
Alkaline secretion from the fundic and antral pouches of the stomach and the loops of proximal and distal duodenum was measured in conscious dogs under basal conditions and after intragastric instillation of HCl solution, meat feeding, or intravenous infusion of various gut hormones. In control tests on fasted dogs HCO-3 output from the duodenal mucosa was severalfold higher than that from the gastric mucosa. Instillation of 10 mM HCl into the stomach resulted in a significant increment in HCO-3 secretion from the gastric pouches and proximal duodenal loops, and this was accompanied by a marked increase in plasma secretin, cholecystokinin (CCK), and pancreatic polypeptide (PP) levels. Meat feeding stimulated HCO-3 secretion from proximal duodenum, and it was accompanied by a significant elevation in plasma gastrin, secretin, CCK, gastric inhibitory peptide, and PP. Among exogenous hormones, the most effective stimulant of HCO-3 secretion was PP, which caused a significant increase in HCO-3 output from the gastric and duodenal mucosa at doses (125-500 pmol X kg-1 X h-1) that raised plasma PP to postprandial levels. CCK in physiological doses (21-85 pmol.kg-.h-1) also stimulated HCO-3 secretion from gastric pouches and proximal duodenal loops. Neurotensin stimulated HCO-3 secretion from both gastric pouches and duodenal loops. In contrast, gastrin or secretin did not affect significantly HCO-3 secretion from the gastroduodenal mucosa. This study provides evidence that some gut hormones, particularly PP, CCK, and neurotensin, may be involved in the physiological stimulation of gastroduodenal alkaline secretion.