Glucose-6-phosphate dehydrogenase plays a pivotal role in nitric oxide-involved defense against oxidative stress under salt stress in red kidney bean
Glucose-6-phosphate dehydrogenase plays a pivotal role in nitric oxide-involved defense against oxidative stress under salt stress in red kidney bean
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红芸豆在盐胁迫下，葡萄糖-6-磷酸脱氢酶在一氧化氮相关的氧化应激防御中发挥着关键作用
DOI：
10.1093/pcp/pcm020
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发表时间：
2007-03-01
影响因子：
4.9
通讯作者：
Bi, Yurong
中科院分区：
文献类型：
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作者：
Liu, Yinggao;Wu, Ruru;Bi, Yurong
研究了红芸豆 (Phaseolus vulgaris) 根中葡萄糖 6-磷酸脱氢酶 (G-6-PDH) 介导的一氧化氮 (NO) 产生在 100 mM 氯化钠诱导的氧化应激耐受性中的关键作用。结果表明，G-6-PDH 活性在 NaCl 存在下迅速增强，并在 100 mM 时达到最大值。 Western blot分析表明，100 mM NaCl处理下红芸豆根中G-6-PDH活性增加主要是由于G-6-PDH蛋白含量增加所致。 100 mM NaCl 还诱导 NO 产生和硝酸还原酶 (NR) 活性。 NaN3（一种 NR 抑制剂）可减少 NO 的产生，但不受 N-omega-硝基-L-精氨酸 (L-NNA)（一种 NOS 抑制剂）的影响。使用 G-6-PDH 抑制剂 2.5 mM Na3PO4 可以阻断 100 mM NaCl 下红芸豆根部 G-6-PDH 和 NR 活性的增加以及 NO 的产生。在 100 mM 氯化钠或硝普钠 (SNP)（NO 供体）存在下，红芸豆根部抗氧化酶的活性增加。在 100 mM NaCl 下测试的所有抗氧化酶的活性增加均被 2.5 mM Na3PO4 完全抑制。基于这些结果，我们得出结论，G-6-PDH 在 NR 依赖性 NO 产生以及建立红芸豆根对盐胁迫的耐受性方面发挥着关键作用。
The pivotal role of glucose-6-phosphate dehydrogenase (G-6-PDH)-mediated nitric oxide (NO) production in the tolerance to oxidative stress induced by 100 mM NaCl in red kidney bean (Phaseolus vulgaris) roots was investigated. The results show that the G-6-PDH activity was enhanced rapidly in the presence of NaCl and reached a maximum at 100 mM. Western blot analysis indicated that the increase of G-6-PDH activity in the red kidney bean roots under 100 mM NaCl was mainly due to the increased content of the G-6-PDH protein. NO production and nitrate reductase (NR) activity were also induced by 100 mM NaCl. The NO production was reduced by NaN3 (an NR inhibitor), but not affected by N-omega-nitro-L-arginine (L-NNA) (an NOS inhibitor). Application of 2.5 mM Na3PO4, an inhibitor of G-6-PDH, blocked the increase of G-6-PDH and NR activity, as well as NO production in red kidney bean roots under 100 mM NaCl. The activities of antioxidant enzymes in red kidney bean roots increased in the presence of 100 mM NaCl or sodium nitroprusside (SNP), an NO donor. The increased activities of all antioxidant enzymes tested at 100 mM NaCl were completely inhibited by 2.5 mM Na3PO4. Based on these results, we conclude that G-6-PDH plays a pivotal role in NR-dependent NO production, and in establishing tolerance of red kidney bean roots to salt stress.