Effect of biological contamination on dentine bond strength of adhesive resins.
Effect of biological contamination on dentine bond strength of adhesive resins.
复制标题
生物污染对粘合树脂牙本质粘合强度的影响。
DOI：
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发表时间：
2003
期刊：
影响因子：
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通讯作者：
S. Botha
中科院分区：
文献类型：
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作者：
J. H. van Schalkwyk;F. Botha;P. J. van der Vyver;F. D. de Wet;S. Botha
本体外研究的目的是确定唾液（S）和血液（B）污染对两种单组分牙本质粘结系统的牙本质粘结强度的影响。120最近提取的咬合三分之一，人磨牙被删除与低速锯，随后嵌入本克尔环通过自固化，丙烯酸树脂。在抛光机中将咬合面在600粒度的碳化硅纸上湿磨，以暴露表面牙本质并产生玷污层。将患牙随机分为12组（n = 10）。所有牙本质表面用34%磷酸蚀刻15秒，用水冲洗，风干3秒，使表面明显湿润。对于对照组（C），根据制造商的说明书，用Scotchbond 1（SB 1，3 M）或Prime & Bond NT（PBNT，Dentsply）处理蚀刻的牙本质表面。在污染组中，通过一次性刷子施加唾液或血液，静置1分钟，然后通过空气喷雾稀释过量的唾液或血液。然后也根据制造商的说明书施加牙本质粘结系统。将复合材料（Z250和TPH）和Compomer（F2000和Dyract AP（D-AP））桩填充并逐渐固化到相应的预处理牙本质表面。所有样本均在37 ℃的水下储存24小时。然后用Zwick试验机以0.5 mm/min的十字头速度对粘结进行应力破坏。在扫描电子显微镜中检查断裂样品。对数据进行统计学分析（Student-t检验）。平均SBS（MPa）为。具有Z250的SB 1：C = 19.1 +/- 4.4; S = 17.3 +/- 3.5; B = 2.6 +/- 0.9;具有F2000的SB 1：C = 11.8 +/- 3.3; S = 9.7 +/- 1.8; B = 4.7 +/- 1.6。含TPH的PBNT：C = 9.2 +/- 3.2; S = 6.5 +/- 3.0; B = 4.3 +/- 1.5;含D-AP的PBNT：C = 10.2 +/- 3.6; S = 9.3 +/- 2.9和B = 7.3 +/- 2.5。两种系统的对照和唾液污染样本之间的剪切粘结强度无统计学显著差异。然而，对照样品和血液污染样品之间的粘合强度存在显著差异。血液污染对粘接系统与牙本质的粘接强度有负面影响。
The purpose of this in vitro study was to determine the effect of saliva (S) and blood (B) contamination on the dentine bond strength of two single-component dentine bonding systems. The occlusal thirds of 120 recently extracted, human molars were removed with a low speed saw and subsequently embedded in Bencor rings by means of self-curing, acrylic resin. The occlusal surfaces were ground wet on 600-grit silicone carbide paper in a polishing machine to expose superficial dentine and to create a smear layer. The teeth were randomly divided into 12 groups (n = 10). All the dentine surfaces were etched with 34% phosphoric acid for 15 seconds rinsed with water, air-dried for 3 seconds, leaving the surfaces visibly moist. For the control groups (C) the etched dentine surfaces were treated with either, Scotchbond 1 (SB1, 3M) or Prime & Bond NT (PBNT, Dentsply) according to the manufacturer's instructions. In the contaminated groups, the saliva or blood was applied by means of a disposable brush, left undisturbed for 1 minute, and the excess then thinned by air spray. The dentine bonding systems were then applied, also according to manufacturer's instructions. Composite (Z250 and TPH) and Compomer (F2000 and Dyract AP (D-AP)) stubs were packed and cured incrementally to the corresponding pretreated dentine surfaces. All specimens were stored for 24 hours under water at 37 degrees C. The bonds were then stressed to failure with a Zwick testing machine, operating at a crosshead speed of 0.5 mm/min. Fractured samples were examined in a Scanning Electron Microscope. The data were statistically analysed (Student-t test). The mean SBS (MPa) were. SB1 with Z250: C = 19.1 +/- 4.4; S = 17.3 +/- 3.5; B = 2.6 +/- 0.9; SB1 with F2000: C = 11.8 +/- 3.3; S = 9.7 +/- 1.8; B = 4.7 +/- 1.6. PBNT with TPH: C = 9.2 +/- 3.2; S = 6.5 +/- 3.0; B = 4.3 +/- 1.5; PBNT with D-AP: C = 10.2 +/- 3.6; S = 9.3 +/- 2.9 and B = 7.3 +/- 2.5. There was no statistical significant difference in shear bond strengths between the control and the saliva-contaminated samples for both systems. There was, however, a significant difference in bond strengths between the control and the blood-contaminated samples. Blood contamination negatively influenced bond strength of bonding systems to dentine.