Genetic variability of Pisolithus isolates associated with native hosts and exotic eucalyptus in the western Mediterranean region.
Genetic variability of Pisolithus isolates associated with native hosts and exotic eucalyptus in the western Mediterranean region.
复制标题
与西地中海地区本地宿主和外来桉树相关的豆豆分离株的遗传变异。
DOI：
10.1046/j.1469-8137.2001.00036.x
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发表时间：
2001
期刊：
影响因子：
9.4
通讯作者：
M. Honrubia
中科院分区：
文献类型：
--
作者：
J. Díez;Berta Anta;J. L. Manjón;M. Honrubia
• 在西地中海地区的外来宿主（桉树）和本地宿主中报告了 Pisolithus 的遗传多样性和宿主特异性。 • 分析了 Pisolithus 核 rDNA 内部转录间隔区 (ITS) 序列的多态性。使用距离和简约方法，将与本地地中海宿主和桉树相关的 17 个分离株的序列与 GenBank DNA 数据库中的序列进行了比较。 • Bootstrap 分析显示所有与地中海宿主相关的 Pisolithus 分离株均聚集在一起。 ITS序列表明存在几种适应不同土壤类型（碱性、酸性和粘土板岩土壤）的生态物种，并对特定的本土宿主具有特异性。来自巴西、肯尼亚和地中海的桉树种植园的分离株与与桉树相关的澳大利亚分离株归为一类。没有发生转移到本机主机；这些外来菌株的宿主特异性范围可能会阻止与当地物种的竞争和杂交。 • Pisolithus spp。地中海盆地的桉树种植园原产澳大利亚；外生菌根真菌的共同引入或许可以解释这些外来森林种植园的成功。
• Genetic diversity and host specificity of Pisolithus is reported here in exotic (Eucalyptus) and native hosts in the western Mediterranean region. • Polymorphism in the internal transcribed spacer (ITS) sequences of the nuclear rDNA of Pisolithus was analysed. Sequences for 17 isolates associated with native Mediterranean hosts and Eucalyptus were compared with those in the GenBank DNA database using distance and parsimony methods. • Bootstrap analysis showed clustering of all Pisolithus isolates associated with Mediterranean hosts. The ITS sequences suggest the occurrence of several ecological species adapted to exploit different soil types (basic, acid and clayey slate-derived soils), with specificity for particular indigenous hosts. Isolates from eucalypt plantations in Brazil, Kenya and the Mediterranean grouped together with eucalypt-associated Australian isolates. Transfer to native hosts did not occur; the host specificity range of these exotic strains might prevent out-competition and interbreeding with local species. • Pisolithus spp. in eucalypt plantations in the Mediterranean basin are of Australian origin; the co-introduction of the ectomycorrhizal fungi might explain the success of these exotic forest plantations.