Optimization of enzyme-assisted negative pressure cavitation extraction of five main indole alkaloids from Catharanthus roseus leaves and its pilot-scale application
Optimization of enzyme-assisted negative pressure cavitation extraction of five main indole alkaloids from Catharanthus roseus leaves and its pilot-scale application
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DOI：
10.1016/j.seppur.2013.12.034
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发表时间：
2014-04-07
影响因子：
8.6
通讯作者：
Zu, Yuan-Gang
中科院分区：
文献类型：
--
作者：
Luo, Meng;Yang, Liu-Qing;Zu, Yuan-Gang
采用新型绿色酶辅助负压空化法（E-NPCE）提取长春花叶中的5种主要吲哚生物碱文多灵（VDL）、长春质碱（CTR）、长春新碱（VCR）、长春碱（VLB）和脱水长春碱（AVLB）。采用Box-Behnken设计（BBD）和响应面法（RSM）对酶解条件进行优化，得到最佳酶解条件为：酶用量0.61%（CB&P，干物质的w/w），pH 4.73，温度35.87 ℃，时间8.62 h。在最佳工艺条件下，VDL、CTR、VCR、VLB和AVLB的提取率分别为4.940 ± 0.215、6.283 ± 0.307、0.049 ± 0.002、0.066 ± 0.003和0.038 ± 0.001 mg/g，比优化前分别提高了38.8%、51.5%、与标准NPCE相比，分别为48.4%、37.5%和58.3%。此外，该提取方法也应用于中试规模提取，其产率分别为4.761 +/- 0.227、6.093 +/- 0.294、0.047 +/- 0.002、0.064 +/- 0.003和0.039 +/- 0.001 mg/g。因此，E-NPCE可以作为天然化合物提取的替代方法，最重要的是该方法可以应用于工业生产。(C)2014爱思唯尔有限公司版权所有。
In this paper, a novel and green enzyme-assisted negative pressure cavitation extraction (E-NPCE) of five main indole alkaloids vindoline (VDL), catharanthine (CTR), vincristine (VCR), vinblastine (VLB) and anhydravinblastine (AVLB) from Catharanthus roseus leaves was investigated. The incubation conditions were studied using Box-Behnken design (BBD) with response surface methodology (RSM), which revealed these as follows: enzyme amount 0.61% (CB&P, w/w of anhydration material), pH 4.73, incubation temperature 35.87 degrees C and time 8.62 h. Under the optimal conditions, the extraction yields of VDL, CTR, VCR, VLB and AVLB reached 4.940 +/- 0.215, 6.283 +/- 0.307, 0.049 +/- 0.002, 0.066 +/- 0.003 and 0.038 +/- 0.001 mg/g, which represented an increase of 38.8%, 51.5%, 48.4%, 37.5% and 58.3%, compared to standard NPCE, respectively. Moreover, this extraction method was also applied on pilot-scale extraction and their yields were 4.761 +/- 0.227, 6.093 +/- 0.294, 0.047 +/- 0.002, 0.064 +/- 0.003 and 0.039 +/- 0.001 mg/g, respectively. Thus, it demonstrated that E-NPCE could be an alternative method for natural compounds extraction and the most important thing is that this method can be applied to industrial produce. (C) 2014 Elsevier B.V. All rights reserved.