Real time electrochemical detection of 5-HT/insulin secretion from single pancreatic islets: Effect of glucose and K+ depolarization
Real time electrochemical detection of 5-HT/insulin secretion from single pancreatic islets: Effect of glucose and K+ depolarization
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DOI：
10.1006/bbrc.1996.1622
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发表时间：
1996-11-01
影响因子：
3.1
通讯作者：
Rosario, LM
中科院分区：
文献类型：
--
作者：
Barbosa, RM;Silva, AM;Rosario, LM
我们报告了一种高灵敏度的电化学方法，适用于从单个胰岛（胰腺中的功能性内分泌单位）释放胰岛素的真实的DME测量。该方法是基于检测的胰岛素替代品5-羟色胺（5-HT）的碳纤维微电极植入胰岛。基于这种新方法与细胞溶质游离Ca 2+浓度（[Ca 2 +]（i））的同时显微荧光记录的组合，我们证明了葡萄糖刺激的胰岛在生理条件下以脉动方式分泌5-HT/胰岛素，并且这种活性由同步[Ca 2 +]（i）振荡编码。检测微量分泌物质变化的灵敏度部分是由示踪剂释放到相对有限的空间（胰岛内间隙）中的事实所赋予的。(C)出版社：Academic Press，Inc.
We report a highly sensitive electrochemical approach suitable for the real dme measurement of insulin release from single islets of Langerhans, the functional endocrine units in the pancreas. The method is based on the detection of the insulin surrogate 5-hydroxytryptamine (5-HT) by carbon fibre microelectrodes implanted in the islets. Based on the combination of this novel approach with the simultaneous microfluorometric recording of cytosolic free Ca2+ concentration ([Ca2+](i)), we demonstrate that glucose-stimulated islets secrete 5-HT/insulin in a pulsatile fashion under physiological conditions and that this activity is encoded by synchronous [Ca2+](i) oscillations. The sensitivity to detect variations in minute amounts of secreted materials is partially conferred by the fact that the tracer is released into a relatively confined space (the intraislet interstitial space). (C) 1996 Academic Press, Inc.