Myofibrillogenesis in skeletal muscle cells
Myofibrillogenesis in skeletal muscle cells
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DOI：
10.1097/01.blo.0000031973.69509.21
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发表时间：
2002-10-01
影响因子：
4.2
通讯作者：
Sanger, JM
中科院分区：
文献类型：
--
作者：
Sanger, JW;Chowrashi, P;Sanger, JM
骨骼肌中的肌原纤维是如何组装的？目前的作者提出了肌原纤维通过三步模型组装的证据：前肌原纤维到新生肌原纤维再到成熟的肌原纤维。这三个步骤的序列最初是基于对活的和固定的心肌培养细胞的研究。来自禽类原代肌肉细胞和转基因骨骼鼠细胞系的数据表明，肌纤维形成的前肌原纤维模型也适用于骨骼肌细胞。前肌原纤维的特征是由由α-肌动蛋白的肌肉异构体组成的Z-小体包围的微型肌节。肌动蛋白细丝连接到这些Z-小体和由非肌肉肌球蛋白II细丝组成的迷你A带。新生的肌原纤维是在肌原纤维排列时形成的，并通过添加菲丁和肌肉肌球蛋白II细丝进行修饰。当非肌球蛋白H从肌原纤维中去除时，当它们之间的距离从前肌原纤维的0.5um增加到成熟肌原纤维的2~2.5um时，成熟的肌原纤维就会产生成熟的肌球蛋白H，富含α-肌动蛋白的Z小体融合。
How are myofibrils assembled in skeletal muscles? The current authors present evidence that myofibrils assemble through a three-step model: premyofibrils to nascent myofibrils to mature myofibrils. This three-step sequence was based initially on studies of living and fixed cultured cells from cardiac muscle. Data from avian primary muscle cells and from a transgenic skeletal mouse cell line indicate that a premyofibril model for myofibrillogenesis also holds for skeletal muscle cells. Premyofibrils are characterized by mini-sarcomeres bounded by Z-bodies composed of the muscle isoform of alpha-actinin. Actin filaments are connected to these Z-bodies and to the mini-A-bands composed of nonmuscle myosin II filaments. Nascent myofibrils are formed when premyotibrils align and are modified by the addition of fitin and muscle myosin II filaments. Mature myofibrils result when nonmuscle myosin H is eliminated from the myofibrils and the alpha-actinin rich Z-bodies fuse as the distance between them increases from 0.5 mum in premyofibrils to 2 to 2.5 mum in the mature myofibrils.