Stereotactic Transcranial Focused Ultrasound Targeting System for Murine Brain Models.
Stereotactic Transcranial Focused Ultrasound Targeting System for Murine Brain Models.
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DOI：
10.1109/tuffc.2020.3012303
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发表时间：
2021-01
期刊：
影响因子：
--
通讯作者：
Xu Z
中科院分区：
文献类型：
--
作者：
Choi SW;Gerhardson TI;Duclos SE;Surowiec RK;Scheven UM;Galban S;Lee FT;Greve JM;Balter JM;Hall TL;Xu Z
提出了一种廉价、精确的聚焦超声立体定向靶向方法，该方法由治疗前 MRI 图像引导，用于小鼠脑模型。分析了立体定向系统每个子组件的不确定性。整个系统使用凝块模型进行校准。该系统的靶向准确性通过体内小鼠胶质母细胞瘤（GBM）模型得到了证明。准确度通过治疗前和治疗后 MR 图像上可见的规定点和消融点之间的绝对距离差来量化。预校准模型研究 (N= 6) 导致轴向、横向和高度轴的误差分别为 0.32 ± 0.31、0.72 ± 0.16 和 1.06 ± 0.38 mm。校准后模型研究 (N= 8) 表明轴向、横向和仰角轴的残余误差分别为 0.09 ± 0.01、0.15 ± 0.09 和 0.47 ± 0.18 mm。在体内 GBM 肿瘤小鼠 (N= 10) 中，校准系统的轴向、横向和高度轴误差显着降低 (p<0.05)，分别为 0.20 ± 0.21、0.34 ± 0.24 和 0.28 ± 0.21 mm。
An inexpensive, accurate focused ultrasound stereotactic targeting method guided by pre-treatment MRI images for murine brain models is presented. Uncertainty of each sub-component of the stereotactic system was analyzed. The entire system was calibrated using clot phantoms. The targeting accuracy of the system was demonstrated with an in vivo mouse glioblastoma (GBM) model. The accuracy was quantified by the absolute distance difference between the prescribed and ablated points visible on the pre- and post-treatment MR images, respectively. A pre-calibration phantom study (N= 6) resulted in an error of 0.32 ± 0.31, 0.72 ± 0.16, and 1.06 ± 0.38 mm in axial, lateral, and elevational axes, respectively. A post-calibration phantom study (N= 8) demonstrated a residual error of 0.09 ± 0.01, 0.15 ± 0.09, and 0.47 ± 0.18 mm in axial, lateral, and elevational axes, respectively. The calibrated system showed significantly reduced (p<0.05) error of 0.20 ± 0.21, 0.34 ± 0.24, and 0.28 ± 0.21 mm in axial, lateral and elevational axes, respectively in the in vivo GBM tumor-bearing mice (N= 10).