Gene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surface
Gene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surface
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DOI：
10.1111/j.1600-0501.2010.02113.x
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发表时间：
2011-04-01
影响因子：
4.3
通讯作者：
Ivanovski, S.
中科院分区：
文献类型：
--
作者：
Donos, N.;Hamlet, S.;Ivanovski, S.
目的 比较人体模型中与中等粗糙度和化学改性的亲水性中等粗糙度表面相关的骨整合基因表达谱。 材料和方法 通过手术将 18 个实心螺钉型圆柱形钛种植体（长 4 毫米、宽 2.8 毫米）、具有中等粗糙度 (SLA) 或化学改性中等粗糙度 (SLActive) 表面插入模型中。 九名人类志愿者的磨牙后区域。愈合后 4、7 和 14 天后，使用环钻将装置取出。收获植入物周围的组织，提取总 RNA，并进行微阵列分析，以确定第 4、7 和 14 天 SLA 和 SLActive 表面之间转录组的差异。结果在第 4 天差异表达的基因列表中没有过度代表的功能相关基因本体类别。然而，到第 7 天，成骨和 SLActive 表面上血管生成相关基因表达上调。成骨和血管生成似乎分别受 BMP 和 VEGF 信号传导的调节。到第 14 天，VEGF 信号在 SLActive 表面上仍然上调，而 BMP 信号在 SLA 表面上上调，这似乎是延迟的补偿反应。此外，神经发生是差异表达基因列表中的一个重要生物过程，并且受到两个表面的影响。结论与 SLA 相比，SLActive 在种植体插入后第 7 天对基因表达产生促骨生成和促血管生成影响，这可能是该表面优越的骨整合特性的原因。引用本文：Donos N、Hamlet S、Lang NP、Salvi GE、Huynh-Ba G、Bosshardt DD、Ivanovski S。亲水性微粗糙种植体表面与疏水性微粗糙种植体表面相比骨整合的基因表达谱。临床。口头实施。资源。 2011 年 22 日； 365-372。
ObjectivesTo compare the gene expression profile of osseointegration associated with a moderately rough and a chemically modified hydrophilic moderately rough surface in a human model.Material and methodsEighteen solid screw-type cylindrical titanium implants, 4 mm long and 2.8 mm wide, with either a moderately rough (SLA) or a chemically modified moderately rough (SLActive) surface were surgically inserted in the retromolar area of nine human volunteers. The devices were removed using a trephine following 4, 7 and 14 days of healing. The tissue surrounding the implant was harvested, total RNA was extracted and microarray analysis was carried out to identify the differences in the transcriptome between the SLA and SLActive surfaces at days 4, 7 and 14.ResultsThere were no functionally relevant gene ontology categories that were over-represented in the list of genes that were differentially expressed at day 4. However, by day 7, osteogenesis- and angiogenesis-associated gene expression were up-regulated on the SLActive surface. Osteogenesis and angiogenesis appeared to be regulated by BMP and VEGF signalling, respectively. By day 14, VEGF signalling remains up-regulated on the SLActive surface, while BMP signalling was up-regulated on the SLA surface in what appeared to be a delayed compensatory response. Furthermore, neurogenesis was a prominent biological process within the list of differentially expressed genes, and it was influenced by both surfaces.ConclusionsCompared with SLA, SLActive exerts a pro-osteogenic and pro-angiogenic influence on gene expression at day 7 following implant insertion, which may be responsible for the superior osseointegrative properties of this surface.To cite this article:Donos N, Hamlet S, Lang NP, Salvi GE, Huynh-Ba G, Bosshardt DD, Ivanovski S. Gene expression profile of osseointegration of a hydrophilic compared with a hydrophobic microrough implant surface.Clin. Oral Impl. Res. 22, 2011; 365-372.