The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-β-D-thiogalactopyranoside exclusion and spectinomycin sensitivity
The Escherichia coli cmlA gene encodes the multidrug efflux pump Cmr/MdfA and is responsible for isopropyl-β-D-thiogalactopyranoside exclusion and spectinomycin sensitivity
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DOI：
10.1128/jb.180.22.6072-6075.1998
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发表时间：
1998-11-01
影响因子：
3.2
通讯作者：
Bouloc, P
中科院分区：
文献类型：
--
作者：
Bohn, C;Bouloc, P
当大肠杆菌CmlA/Cmr/MdfA外排泵过表达时，来自异丙基-β-D-硫代半乳糖苷（IPTG）调节的启动子的克隆基因的表达降低，这可能是由于IPTG从细胞质中排除。先前报道的cmlA 1突变赋予了类似的表型，cmlA 1在cmr/mdfA上游含有IS 30插入，其产生了推定的启动子。CmlA的过度产生也会导致大观霉素过敏。
Expression of cloned genes from isopropyl-beta-D-thiogalactopyranoside (IPTG)-regulated promoters is lowered when the Escherichia coli CmlA/Cmr/MdfA efflux pump is is overexpressed, probably due to IPTG exclusion from the cytoplasm. The previously reported cmlA1 mutation confers a similar phenotype, cmlA1 contains an IS30 insertion upstream of cmr/mdfA, which creates a putative promoter. CmlA overproduction also causes spectinomycin hypersensitivity.