Mitochondrial genomes and 28S rDNA contradict the proposed obsoletion of the order Tetraonchidea (Platyhelminthes: Monogenea)
Mitochondrial genomes and 28S rDNA contradict the proposed obsoletion of the order Tetraonchidea (Platyhelminthes: Monogenea)
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线粒体基因组和 28S rDNA 与 Tetraonchidea 目（扁形动物：Monogenea）的废弃提议相矛盾
DOI：
10.1016/j.ijbiomac.2019.09.150
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发表时间：
2020
影响因子：
8.2
通讯作者：
Gui T.Wang
中科院分区：
文献类型：
--
作者：
Dong Zhang;Wen X.Li;Hong Zou;Shan G.Wu;Ming Li;Ivan Jakovlić;Jin Zhang;Rong Chen;Gui T.Wang
由于基于形态的假设的不一致和分子数据的缺乏，四轮纲的有效性仍然存在争议。目前唯一可用于整个目的完整线粒体基因组是 Paratetraonchoides inermis（Tetraonchoididae）。为了从线粒体基因组学的角度研究Tetraonchidea的系统发育，我们对Tetraonchidae科：Tetraonchus monenteron（Tetraonchidea）的第一个线粒体基因组进行了测序。为了获得核数据的视角，我们还对两个物种的核 28SrDNA 基因进行了测序。 T 的有丝分裂基因组。 monenteron 不具有高 A + T 含量，也不具有 tRNA 假基因，这两者都是 P 中报道的独特特征。无刺。然而，T. monenterone 表现出独特的基因顺序，与 P 相比有大量 tRNA 重排。 inermis和其他单殖动物。使用贝叶斯推理和最大似然方法进行的系统发育分析，辅以分区，一致支持 T 的姐妹群关系。 monenteron (Tetraonchidae) 和 P.无刺（Tetraonchoididae）。 28SrDNA 数据和两个形态学无源性也部分支持了这一点。 Tetraonchidae 和 Tetraonchoididae 的这种密切关系对最新的基于形态学的主要分类提出了挑战，该分类提出了 Tetraonchidea 目的废弃，并将 Tetraonchoididae 归入 Gyrodactylidea 分支。该命令的有效性还需更多数据进一步证实。
Due to the incongruence of morphology-based hypotheses and scarcity of molecular data, validity of the order Tetraonchidea remains contentious. The only complete mitogenome currently available for the entire order is that ofParatetraonchoides inermis(Tetraonchoididae). To study the phylogeny of Tetraonchidea from mitogenomic perspective, we sequenced the first mitogenome for the family Tetraonchidae:Tetraonchus monenteron(Tetraonchidea). To get a nuclear-data perspective, we also sequenced nuclear28SrDNA gene of both species. The mitogenome ofT. monenterondoes not have high A + T content, nor tRNA pseudo-genes, both of which were unique features reported inP. inermis. However,T. monenteronexhibits a unique gene order, with a large number of tRNA rearrangements in comparison toP. inermisand other monogeneans. Phylogenetic analyses conducted using Bayesian inference and maximum likelihood methods, complemented with partitioning, consistently support the sister-group relationship ofT. monenteron(Tetraonchidae) andP. inermis(Tetraonchoididae). This is also partially supported by the28SrDNA data and two morphologic apomorphies. This close relationship of Tetraonchidae and Tetraonchoididae challenges the latest major morphology-based classification, which proposed obsoletion of the Tetraonchidea order, and grouped Tetraonchoididae into the Gyrodactylidea clade. The validity of this order shall have to be further confirmed with more data.