Soil metabolome correlates with bacterial diversity and co -occurrence patterns in root -associated soils on the Tibetan Plateau
Soil metabolome correlates with bacterial diversity and co -occurrence patterns in root -associated soils on the Tibetan Plateau
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DOI：
10.1016/j.scitotenv.2020.139572
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发表时间：
2020-09-15
影响因子：
9.8
通讯作者：
Wang, Jianjun
中科院分区：
文献类型：
--
作者：
Liu, Kaihui;Ding, Xiaowei;Wang, Jianjun
根区土壤中的代谢物调节微生物与微生物之间的相互作用，并控制整个微生物群落。然而，化学物质如何与根相关土壤中细菌群落的多样性和共生模式相关仍然知之甚少。在这里，我们研究了青藏高原不同土地类型根际土壤中细菌群落多样性和共生模式与土壤代谢组的关系。土壤代谢物主要包括一系列有机酸、糖和糖衍生物，它们与细菌α多样性呈广泛的负相关。与调查的环境变量相比，代谢物对香农多样性和细菌群落组成的影响更大。与糖相比，有机酸在较高分类级别的细菌群落组成中所占比例更大，而在属和种水平上则相反。一些细菌属和代谢物的相对丰度与土壤类型和植物基因密切相关。这些差异化合物与不同土地类型和不同植物类型的不同细菌分类群显著相关。共生网络中的关键物种，如慢生根瘤菌、短杆菌和微病毒杆菌，与糖和有机酸之间存在显著的相关性。结构方程模型表明，糖代谢在改变细菌群落多样性方面起着至关重要的作用。这项研究为青藏高原根际土壤细菌群落维持的生态机制提供了新的见解。
Metabolites in root-zone soils mediate microbe-to-microbe interactions and govern the overall microbial community. However, how chemicals relate to diversity and co-occurrence patterns of bacterial communities in root-associated soils is still poorly understood. Here, we studied the relationships of soil metabolome with bacterial community diversity and co-occurrence patterns in root-associated soils across different land types on the Tibetan Plateau. The soil metabolome mainly encompassed a range of organic acids, and sugars and sugar derivatives, which were widely negatively correlated with bacterial alpha-diversity. Compared to the investigated environmental variables, metabolites accounted more for the variations in the Shannon diversity and bacterial community compositions. Compared to sugars, organic acids accounted more for bacterial community compositions at high taxonomic ranks, while reversed at genus and species levels. The relative abundances of some bacterial genera and metabolites were closely linked to soil types and plant genotypes. The differential compounds were significantly correlated with the distinctive bacterial taxa across land types and plant genotypes. Keystone species in co-occurrence network, such as Bradyrhizobium, Bryobacter, and Microvirga were significantly correlated with sugars and organic acids. Structural equation modeling revealed that sugar metabolism can play a crucial role in altering the bacterial community diversity. This study provides new insights into the ecological mechanism that maintains bacterial community in the root-associated soils on the Tibetan Plateau.