D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut.
D-Hillarin, a novel W180-domain protein, affects cytokinesis through interaction with the septin family member Pnut.
复制标题
D-Hillarin 是一种新型 W180 结构域蛋白，通过与 septin 家族成员 Pnut 相互作用影响胞质分裂。
DOI：
10.1002/neu.20131
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Johansen,Jørgen
中科院分区：
文献类型：
--
作者：
Ji,Yun;Rath,Uttama;Girton,Jack;Johansen,KristenM;Johansen,Jørgen
By database searches of theDrosophilagenome project we have identified D‐hil as the fly member of a novel family of W180‐domain containing proteins. Immunocytochemistry demonstrated that D‐hil is localized to the neuropil of the embryonic CNS, to the cellular cortex of dividing neuroblasts from larval brains, and that it is up‐regulated in the cleavage furrow of S2 cells. We show that D‐hil distribution overlaps extensively with that of the septin family member Pnut. Cross‐immunoprecipitation experiments further indicated that the two proteins may be members of the same protein complex. Analysis of a severe hypomorphic P‐element mutation in theD‐hillocus suggested that D‐hil is a nonessential protein. However, by creating double mutant flies we show that theD‐hillocus acts as a modulator of Pnut function by increasing the level of polyploidy of neuroblasts in PnutKG00478/PnutKG00478larval brains. Based on these results we propose that D‐hil may function as a regulator of septin function during cytokinesis in the developing nervous system. © 2005 Wiley Periodicals, Inc. J Neurobiol, 2005
