Fluid flow simulations verified by measurements to investigate adsorption processes in a static mixer
Fluid flow simulations verified by measurements to investigate adsorption processes in a static mixer
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通过测量验证流体流动模拟，以研究静态混合器中的吸附过程
DOI：
10.1016/j.camwa.2018.08.066
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
M. J. Krause
中科院分区：
文献类型：
--
作者：
M.-L. Maier;S. Milles;S. Schuhmann;G. Guthausen;H. Nirschl;M. J. Krause
蛋白质纯化是一项复杂的任务，因为靶蛋白必须从副产品中分离出来。一种提取蛋白质（称为吸附）的方法是将吸附过程与高梯度磁分离相结合。这种机制包括吸附剂在磁性颗粒的功能化表面上的吸附和随后的磁力分离。为了通过模拟深入了解吸附过程，进行了适当的研究。在这项工作中，三组分流（水，吸附剂，颗粒）是由一种新的欧拉-欧拉-拉格朗日方法来描述的，该方法考虑了悬浮在水中的移动颗粒的吸附过程。由于吸附受水流和流体混合的影响，在静态混合器中进行了模拟，并通过测量进行了验证。证明了吸附法的可行性。水的流动采用Navier-Stokes方程，吸附采用平流-扩散-反应方程。假设粒子为点粒子，采用基于牛顿运动方程的离散粒子方法进行描述。用晶格玻尔兹曼方法计算流体。在吸附剂的情况下，引入汇项的公式来考虑吸附。通过对水流速度的磁共振成像测量，验证了水流的模拟结果，结果吻合得很好。为了验证流体混合模拟的正确性，对水和油墨溶液进行了实际实验。两者在混合器中表现出相似的浓度分布。提出了考虑水中悬浮颗粒吸附过程的方法的可行性。
Protein purification is a complex task as the target proteins have to be isolated from the byproducts. One way to extract the proteins (referred to as adsorptive) is to combine adsorption processes and a high-gradient magnetic separation. This mechanism involves the adsorption of the adsorptive onto the functionalized surface of a magnetic particle and its subsequent separation by magnetic forces. To get insight into the adsorption processes by simulation, appropriate investigations are performed. In this work, the three-component flow (water, adsorptive, particles) is described by a novel Euler–Euler–Lagrange approach that considers adsorption processes on the moving particles suspended in water. As the adsorption is influenced by the water flow and the fluid mixing, the simulations are conducted in a static mixer and verified by measurements. The feasibility of the adsorption approach is shown.The water flow is modeled by the Navier–Stokes equations, and the adsorptive by the advection–diffusion–reaction equation. The particles are assumed to be point particles and are described with a discrete particle method based on Newton’s equation of motion. The fluids are computed using a lattice Boltzmann method. In case of the adsorptive, the formulation of the sink term is introduced to consider the adsorption.The simulation of the water flow is verified by magnetic resonance imaging measurements of the velocity, and the results show very good accordance. To justify the fluid mixing simulation, a real experiment with water and an ink solution is conducted. Both show a similar concentration profile in the mixer. The feasibility of the approach considering the adsorption processes on in water suspended particles is presented.
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DOI：
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发表时间：
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