Selection of a synthetic glycan oligomer from a library of DNA-templated fragments against DC-SIGN and inhibition of HIV gp120 binding to dendritic cells
Selection of a synthetic glycan oligomer from a library of DNA-templated fragments against DC-SIGN and inhibition of HIV gp120 binding to dendritic cells
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DOI：
10.1039/c1cc13213j
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发表时间：
2011-01-01
影响因子：
4.9
通讯作者：
Winssinger, Nicolas
中科院分区：
文献类型：
--
作者：
Ciobanu, Mihai;Huang, Kuo-Ting;Winssinger, Nicolas
我们报告了核酸编码的碳水化合物库的合成、其组合自组装成37485对以及针对DC-SIGN的筛选，从而鉴定了共有配体基序。来自所选对的原型实例显示具有增强的结合。一个树枝状聚合物纳入选定的基序抑制gp 120的树突状细胞的结合效率高于甘露聚糖。
We report the synthesis of a nucleic acid-encoded carbohydrate library, its combinatorial self-assembly into 37 485 pairs and a screen against DC-SIGN leading to the identification of consensus ligand motifs. A prototypical example from the selected pairs was shown to have enhanced binding. A dendrimer incorporating the selected motifs inhibited gp120's binding to dendritic cells with higher efficiency than mannan.