Analysis of flavonoids in flower petals of soybean near-isogenic lines for flower and pubescence color genes
Analysis of flavonoids in flower petals of soybean near-isogenic lines for flower and pubescence color genes
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DOI：
10.1093/jhered/esm012
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发表时间：
2007-05-01
影响因子：
3.1
通讯作者：
Takahashi, Ryoji
中科院分区：
文献类型：
--
作者：
Iwashina, Tsukasa;Githiri, Stephen M.;Takahashi, Ryoji
W1、W3、W4和Wm基因控制花的颜色，T和Td基因控制短柔毛的颜色。W1、W3、Wm和t4分别编码类黄酮3′5′-羟化酶（EC 1.14.13.88）、二氢黄酮醇4-还原酶（EC 1.1.1.219）、类黄酮醇合酶（EC 1.14.11.23）和类黄酮3′-羟化酶（EC 1.14.13.21）。本研究的目的是测定花瓣中原花青素、黄酮醇和clihydro黄酮醇的结构。紫花品种Clark （W1W1 w3w3 W1W1 WmWm TT TdTd）和Hato大豆（W1W1 w3w3 W1W1 WmWm TT TdTd）花青素的主要成分为malvidin 3,5-二- o -葡萄糖苷，次要成分为delphiniclin 3,5-二- o -葡萄糖苷。一级黄酮醇为山奈酚3- o -龙胆苷，二氢黄酮醇为芳香腺苷3- o -葡萄糖苷。对花或短柔毛颜色基因Clark-w1（白花）、Clark-w4（近白花）、Clark-W3w4（淡紫色花）、Clark-t（灰色短柔毛）、Clark-td（近灰色短柔毛）、Harosoy-wm（洋红色花）和Harosoy-T（茶色短柔毛）的近等基因系（NILs）进行了定量分析。在Clark-w1和Clark-w4中未检测到花青素，而在Clark-W3w4中检测到微量花青素。含有w1和w4的NILs中黄酮醇和二氢黄酮醇的含量与含有紫色花的NILs基本相似，表明w1和w1仅影响花青素的生物合成。在Harosoy-wm中，黄酮醇苷的含量大幅减少，而clihydroflavonoids ol的含量增加，这表明Wm参与了clihydroflavonoids ol生成黄酮醇的过程。隐性mim等位基因减少了黄酮醇的数量，抑制了花青素和黄酮醇之间的共色素沉着，导致花的颜色变蓝（洋红色）。短柔毛颜色基因Tor Td对花瓣类黄酮合成无明显影响。
W1, W3, W4, and Wm genes control flower color, whereas T and Td genes control pubescence color in soybean. W1, W3, Wm, and Tare presumed to encode flavonoid 3'5'-hydroxylase (EC 1.14.13.88), dihydroflavonol 4-reductase (EC 1.1.1.219), flavonol synthase (EC 1.14.11.23), and flavonoid 3'-hydroxylase (EC 1.14.13.21), respectively. The objective of this study was to determine the structure of the primary anthocyanin, flavonol, and clihydroflavonol in flower petals. Primary component of anthocyanin in purple flower cultivars Clark (W1W1 w3w3 W1W1 WmWm TT TdTd) and Hato soy (W1W1 w3w3 W1W1 WmWm tt TdTd) was malvidin 3,5-di-O-glucoside with delphiniclin 3,5-di-O-glucoside as a minor compound. Primary flavonol and dihydroflavonol were kaempferol 3-O-gentiobioside and aromadendrin 3- O-glucoside, respectively. Quantitative analysis of near-isogenic lines (NILs) for flower or pubescence color genes, Clark-w1 (white flower), Clark-w4 (near-white flower), Clark-W3w4 (dilute purple flower), Clark-t (gray pubescence), Clark-td (near-gray pubescence), Harosoy-wm (magenta flower), and Harosoy-T(tawny pubescence) was carried out. No anthocyanins were detected in Clark-w1 and Clark-w4, whereas a trace amount was detected in Clark-W3w4. Amount of flavonols and dihydroflavonol in NILs with w1 or w4 were largely similar to the NILs with purple flower suggesting that W1 and W1 affect only anthocyanin biosynthesis. Amount of flavonol glycosides was substantially reduced and clihydroflavonol was increased in Harosoy-wm suggesting that Wm is responsible for the production of flavonol from clihydroflavonol. The recessive mim allele reduces flavonol amount and inhibits co-pigmentation between anthocyanins and flavonols resulting in less bluer (magenta) flower color. Pubescence color genes, Tor Td, had no apparent effect on flavonoid biosynthesis in flower petals.