Role of Spinal Cord in Regulating Mouse Kidney: A Virally Mediated Trans-synaptic Tracing Study
Role of Spinal Cord in Regulating Mouse Kidney: A Virally Mediated Trans-synaptic Tracing Study
复制标题
脊髓在调节小鼠肾脏中的作用：病毒介导的跨突触追踪研究。
DOI：
10.1016/j.urology.2011.11.003
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发表时间：
2012-03-01
期刊：
影响因子：
2.1
通讯作者：
Zhang, Xu
中科院分区：
文献类型：
--
作者：
Ye, Da-Wei;Li, Rong-Chun;Zhang, Xu
目的研究脊髓的神经支配和神经元联系对研究肾代谢反应具有重要意义。材料与方法采用伪狂犬病病毒（PRV）-614对10只C57 BL/6 J系成年雄性小鼠脊髓进行逆行定位。结果注射PRV-614后第5天，在脊髓T4-L1节段的中间外侧细胞柱、闰核或中央自主神经核中发现PRV-614阳性细胞，在脊髓T4-L1节段的中间外侧细胞柱、闰核和中央自主神经核中发现PRV-614阳性细胞，在脊髓T4-L1节段的中间外侧细胞柱、闰核和中央自主神经核中发现PRV-614阳性细胞，在脊髓T4-L1节段的中间外侧细胞柱、中央自主神经核和中央自主神经核中发现PRV-614阳性细胞，在脊髓T4-L1节段的中间外侧细胞柱、闰核和中央自主神经核中发现PRV-614阳性细胞。PRV-614标记细胞主要分布在T9节段。结论本研究揭示了肾脏与脊髓中间外侧细胞柱神经元之间的神经解剖回路。泌尿学79：745.e1-745.e4，2012年。(C)2012 Elsevier Inc.
OBJECTIVE To determine the spinal innervation and neuronal connections is important for studying renal metabolic responses.MATERIALS AND METHODS In this study, the spinal cords of 10 adult male C57BL/6J strain mice were mapped retrograde using injections of pseudorabies virus (PRV)-614. The virus, injected into the kidney, was specifically transported to the spinal cord.RESULTS At 5 days after injection of the PRV-614, PRV-614-positive cells were found in the intermediolateral cell column, the intercalates nucleus, or the central autonomic nucleus of spinal cord segments T4-L1, and most PRV-614-labeled cells were found in the T9 segment.CONCLUSION Our results revealed neuroanatomical circuits between kidney and the spinal intermediolateral cell column neurons. UROLOGY 79: 745.e1-745.e4, 2012. (C) 2012 Elsevier Inc.