Enzyme-Linked Immunosorbent Assays Based on Neospora caninum Dense Granule Protein 7 and Profilin for Estimating the Stage of Neosporosis
Enzyme-Linked Immunosorbent Assays Based on Neospora caninum Dense Granule Protein 7 and Profilin for Estimating the Stage of Neosporosis
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DOI：
10.1128/cvi.05669-11
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发表时间：
2012-03-01
影响因子：
--
通讯作者：
Nishikawa, Yoshifumi
中科院分区：
文献类型：
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作者：
Hiasa, Jun;Nishimura, Maki;Nishikawa, Yoshifumi
犬新孢子虫（Neospora caninum）是一种引起牛和犬新孢子虫病的细胞内原生动物寄生虫，其特征分别为胎儿流产和新生儿死亡以及神经肌肉麻痹。虽然已经报道了许多检测寄生虫特异性抗体或寄生虫DNA的诊断方法，但迄今为止还没有描述用于估计病理状态的有效血清学诊断技术。我们的研究旨在阐明寄生虫特异性抗体反应、寄生虫激活和N。犬感染，通过使用重组抗原为基础的酶联免疫吸附试验。在实验感染的小鼠中，抗N.犬前纤维蛋白（NcPF）抗体仅在神经系统症状动物中检测到。有症状小鼠脑内的寄生虫数量显著高于无症状动物。此外，抗NcPF和抗NcGRA7抗体主要在实验感染犬的急性期检测到，而抗NcSAG1抗体在急性期和慢性期均产生。此外，在抗NcSAG1抗体阳性的临床犬中，有神经系统症状的犬的抗NcGRA7和抗NcPF抗体的阳性率显著高于无神经系统症状的动物。我们的研究结果表明，抗NcGRA7和抗NcPF抗体的水平反映了寄生虫激活和犬的神经症状。总之，抗NcGRA7和NcPF的抗体可能具有作为估计新孢子虫病的病理状态的合适指标的潜力。
Neospora caninum is an intracellular protozoan parasite that causes bovine and canine neosporosis, characterized by fetal abortion and neonatal mortality and by neuromuscular paralysis, respectively. Although many diagnostic methods to detect parasite-specific antibodies or parasite DNA have been reported, to date no effective serodiagnostic techniques for estimating pathological status have been described. Our study aimed to elucidate the relationship between the parasite-specific antibody response, parasite activation, and neurological symptoms caused by N. caninum infection by using a recombinant antigen-based enzyme-linked immunosorbent assay. Among experimentally infected mice, anti-N. caninum profilin (NcPF) antibody was only detected in neurologically symptomatic animals. Parasite numbers within the brains of the symptomatic mice were significantly higher than those in asymptomatic animals. In addition, anti-NcPF and anti-NcGRA7 antibodies were mainly detected at the acute stage in experimentally infected dogs, while anti-NcSAG1 antibody was produced during both acute and chronic stages. Furthermore, among anti-NcSAG1 antibody-positive clinical dogs, the positive rates of anti-NcGRA7 and anti-NcPF antibodies in the neurologically symptomatic dogs were significantly higher than those in the non-neurologically symptomatic animals. Our results suggested that the levels of anti-NcGRA7 and anti-NcPF antibodies reflect parasite activation and neurological symptoms in dogs. In conclusion, antibodies against NcGRA7 and NcPF may have potential as suitable indicators for estimating the pathological status of neosporosis.