Contrast expression patterns of JNK1 during sex reversal of the rice-field eel.
Contrast expression patterns of JNK1 during sex reversal of the rice-field eel.
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DOI：
10.1002/jez.b.21332
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发表时间：
2009-11
期刊：
影响因子：
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通讯作者：
Ya Xiao;Lili Chen;Jiao Liu;Wen-bin Liu;He-ge Chen;Li-Jun Zou;Yun Liu;David Wan-cheng Li
中科院分区：
文献类型：
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作者：
Ya Xiao;Lili Chen;Jiao Liu;Wen-bin Liu;He-ge Chen;Li-Jun Zou;Yun Liu;David Wan-cheng Li
c-Jun N-末端激酶（JNK）是丝裂原活化蛋白激酶家族的成员。它们在调节动物发育方面的功能在无脊椎动物和脊椎动物中都得到了很好的研究。然而，它们是否在性别决定中发挥作用仍有待确定。在这里，我们提出的第一个证据表明，JNK 1的表达显示不同的模式在性逆转的黄鳝。分子克隆显示JNK 1在黄鳝等脊椎动物中具有很高的保守性。定量实时聚合酶链反应和Western印迹分析表明，JNK 1是高度表达的雌性个体的卵巢和减少到一个相当大的程度，在后期阶段的间性。然而，当中间性个体发育到雄性阶段时，JNK 1在雄性个体睾丸中的表达明显下调。与雌性和雄性性腺中JNK 1表达模式的对比相关，包括Nanog，Oct-3/4和Sox-2在内的几种干细胞标记基因也在雌性和雄性生殖干细胞中差异表达。总之，这些结果表明JNK 1可能在黄鳝的性逆转中起重要作用。
The c-Jun N-terminal kinases (JNKs) are members of the mitogen-activated protein kinase family. Their functions in regulating animal development have been well studied in both invertebrates and vertebrates. However, it remains to be determined whether they play a role in sex determination. Here we present first evidence to show that expression of JNK1 displays distinct patterns during sex reversal of rice-field eel. Molecular cloning reveals that JNK1 is well conserved among rice-field eel and other vertebrates. Both quantitative real-time polymerase chain reaction and Western blot analysis demonstrate that JNK1 is highly expressed in the ovary of the female individual and reduced to a substantial degree at the later stage of the intersex. However, when the intersex individual develops into the stage of male, expression of the JNK1 in the testis of the male individual is distinctly downregulated. Associated with the contrast JNK1 expression pattern in female and male gonads, several stem cell marker genes including Nanog, Oct-3/4, and Sox-2 were also differentially expressed in female and male germinal stem cells. Together, these results suggest it is possible that JNK1 plays an important role in sexual reversal of the rice-field eel.