A packaging technique for polymer microfluidic platforms
A packaging technique for polymer microfluidic platforms
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DOI：
10.1021/ac034990t
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发表时间：
2004-02-15
影响因子：
7.4
通讯作者：
Lee, LJ
中科院分区：
文献类型：
--
作者：
Lai, SY;Cao, X;Lee, LJ
发展了一种新的聚合物微流控器件键合和表面改性技术--树脂-气体注入法。这种方法可以很容易地键合具有复杂流动模式的生物芯片。对级联式微混合器和多通道DNA测序芯片进行了实验验证。通过添加表面改性剂，可以控制基底与水的界面自由能。通道表面的局部改性也可以通过连续的树脂-气体注入结合掩蔽技术来实现。在应用中，该技术用于在微通道的壁上形成干燥的整体固定水凝胶层，用作电泳分离DNA片段的筛分材料。实验比较了该技术与传统的线性聚合物溶液法在基于微通道的DNA测序过程中的试剂装载量和电泳分离效率。实验结果表明，该方法操作简便，上样速度快，但分离效率稍低。
A new technique, resin-gas injection, has been developed for bonding and surface modification of polymer microfluidic devices. This method can easily bond biochips with complex flow patterns. A cascade micromixer and a multichannel DNA sequencing chip were demonstrated experimentally. By adding surface modification agents, the interfacial free energy of the substrate with water can be controlled. Local modification of the channel surface can also be achieved through sequential resin-gas injection in conjunction with a masking technique. For application, this technique is used to form a layer of dry monolithic stationary hydrogel on the walls of a microchannel, serving as a sieving material for electrophoresis separation of DNA fragments. The reagent loading and the electrophoresis separation efficiency of this technique were compared experimentally with the conventional linear polymer solution method used in the microchannel-based DNA sequencing process. It is found that our method has the advantages of more user-friendly operation, easier and faster sample loading, but slightly less separation efficiency.