ORIGIN OF REPLICATION, ORIC, OF THE ESCHERICHIA-COLI-K12 CHROMOSOME - GENETIC-MAPPING AND MINICHROMOSOME REPLICATION
ORIGIN OF REPLICATION, ORIC, OF THE ESCHERICHIA-COLI-K12 CHROMOSOME - GENETIC-MAPPING AND MINICHROMOSOME REPLICATION
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DOI：
10.1101/sqb.1979.043.01.018
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
MESSER, W
中科院分区：
文献类型：
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作者：
VONMEYENBURG, K;HANSEN, FG;MESSER, W
大肠杆菌环状染色体的复制（凯恩斯1964）是双向的（Masters and Broda 1971; Bird et al. 1972; Prescott and Kuempel 1972）。复制似乎起始于一个固定位点（Marsh和Worcel 1977），该位点位于ilv操纵子逆时针方向约20兆道尔顿（MD）处（von Meyenburg等，1977），在遗传图谱上的82.5分钟处（Bachmann等，1976）。对于该位点，染色体的复制起点，引入了符号oriC（Hiraga 1976）。在这里，我们回顾了我们的方法，oriC的遗传作图，通过分离专门的转导Aasn携带oriC和他们的建立作为微型染色体（冯Meyenburg等，1978年）。我们描述了由E. coli染色体DNA。此外，我们讨论了初步尝试，以表征在体内复制和分区的微型染色体，以及它们对宿主的生长生理的影响。
Replication of the circular chromosome of Escherichia coli (Cairns 1964) is bidirectional (Masters and Broda 1971; Bird et al. 1972; Prescott and Kuempel 1972). Replication appears to be initiated at a fixed site (Marsh and Worcel 1977) which has been located about 20 megadaltons (MD) counterclockwise of the ilv operon (von Meyenburg et al. 1977) at 82.5 minutes on the genetic map (Bachmann et al. 1976). For this site, the origin of replication of the chromosome, the symbol oriC has been introduced (Hiraga 1976). Here we review our approach to the genetic mapping of oriC through isolation of specialized transducing phages Aasn carrying oriC and their establishment as minichromosomes (von Meyenburg et al. 1978). We describe the construction of minichromosomes consisting exclusively of E. coli chromosomal DNA. In addition, we discuss initial attempts to characterize the in vivo replication and partitioning of minichromosomes, as well as their effects on the growth physiology of the host.