Genomic Variation in Micropropagated Robinia ambigua `idahoensis' Revealed by RAPD Markers
Genomic Variation in Micropropagated Robinia ambigua `idahoensis' Revealed by RAPD Markers
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RAPD 标记揭示微繁殖刺槐“爱达荷州”的基因组变异
DOI：
10.21273/hortsci.41.6.1466
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发表时间：
2006
期刊：
影响因子：
1.9
通讯作者：
Yingshan Dong
中科院分区：
文献类型：
--
作者：
F. Ngezahayo;Wanli Guo;L. Gong;Fang;Bao Liu;Yingshan Dong
作者先前报道了一种高效的刺槐(IDAHOUS‘IDAHOUS’)体外大规模繁殖系统。他们的方法使用了来自单一供体植物的腋芽的增强分枝，以及通过ISSR标记检测体细胞无性系变异。由于ISSR往往聚集在植物基因组中特定的染色体区域，因此基因组变异的程度和范围以及变异背后的序列值得进一步研究。在本研究中，作者利用一种更通用的分子标记--随机扩增多态性DNA(随机扩增多态性DNA)，对同一组随机选取的40株可疑大麦的微繁殖植株进行了34个引物的分析。此外，他们还对一些可变条带进行了测序。在获得的260条可重复扩增的条带中，有70条在41株植株(40株微繁植株和1株供体植株)中表现出多态，多态水平为26%。聚类分析表明，遗传相似度在0.61~0.97之间。在这些变异背后的20条测序带中，有8条与已知或预测的功能细胞基因或逆转录元件显示出显著的同源性。这些结果清楚地表明，即使是腋芽的分枝增强，也可以伴随着广泛的基因组变异，这一点在利用试管技术进行商业化繁殖时应加以考虑。刺槐是豆科(豆科)刺槐属植物，俗称爱达荷州刺槐，可能是刺槐(R.puoacacia L.)灌木刺槐(R.hispida L.)或粘性蝗虫(R.visa vent.)这棵树具有两个亲本物种的综合特征，包括更高的植物高度(15米比9-30米)和刺槐鲜艳的色彩(Fowells，1965)。通过体外培养进行微繁殖已被证明是繁殖濒危或有价值植物的有效手段(Osorio-Rosales和Mata-Rosas，2005；Par-Edes和Lavin，2005)。最近，我们利用腋芽的增强型分枝建立了一种高效的多疑红景天的体外培养体系(Guo et al.，2006)。令人惊讶的是，通过ISSR标记进行的分子分析显示了这些繁殖植物的基因组变异(Guo等人，2006年)。然而，由于ISSR标记通常局限于特定的染色体区域，如植物基因组中的抗病基因类似簇(Ratnparkhe等人，1998)，基因组变异的程度和范围以及这些变异背后的序列仍然未知。这项研究的目的是通过使用一种更通用的分子标记--随机扩增多态DNA(随机扩增多态性DNA)来解决这些问题，该标记已知在整个基因组中分布和扩增无偏倚基因座(Sauve等人，2005年；Williams等人，1990年)。我们报告说，在双歧杆菌中确实发生了高水平的基因组变异。伊达荷斯的微繁植株。此外，细胞基因和逆转录因子似乎是这些变异的次要原因。
The authors have previously reported an efficient in vitro system for mass micropropagation of Robinia ambigua 'idahoensis' (Idaho locust). Their method used enhanced branching of axillary buds from a single donor plant along with detection of somaclonal variation by the intersimple sequence repeat (ISSR) markers. Because ISSRs tend to be clustered to specific chromosomal regions in plant genomes, the extent and scope of the genomic variations and the sequences underlying the variation warranted further investigations. In this study, the authors analyzed the same set of 40 randomly selected micropropagated R. ambigua plants by a more general molecular marker— random amplified polymorphic DNA (RAPD) using 34 selected primers. In addition, they sequenced some of the variable bands. Of the 260 reproducible RAPD bands scored, 70 were polymorphic among the 41 plants (40 micropropagated and one donor), corresponding to a polymorphism level of 26%. Cluster analysis revealed a genetic similarity ranging from 0.61 to 0.97. Of the 20 sequenced bands underlying the variations, eight showed significant homology to known or predicted functional cellular genes or retroelements. These results clearly indicated that micropropagation of R. ambigua, even by enhanced branching of axillary buds, can be accompanied by extensive genomic variations, which should be taken into account for commercial propagation of this plant by in vitro means. Robinia ambigua, commonly called Idaho locust, belongs to the legume family (Fabaceae), and is presumably a hybrid be- tween the black locust (R. pseudoacacia L.) and either the shrub bristly locust (R. hispida L.) or the clammy locust (R. viscosa Vent.). This tree has combined features of both parental species, including the higher plant stature of the black locust (15 m compared with 9-30 m) and the vivid coloring of the bristly locust (Fowells, 1965). Micropropagation through in vitro culture has been shown to be an efficient means for propagation of endangered or valuable plants (Osorio-Rosales and Mata-Rosas, 2005; Par- edes and Lavin, 2005). Recently, we have established an efficient in vitro culture system for the micropropagation of R. ambigua by using enhanced branching of axillary buds (Guo et al., 2006). Surprisingly, molecular analysis by the intersimple sequence repeat (ISSR) markers showed genomic variations in these propagated plants (Guo et al., 2006). Nonetheless, because the ISSR markers often confine to specific chromosomal regions, such as the disease-resistance gene analogous clusters (Ratnaparkhe et al., 1998), in plant genomes, the extent and scope of the genomic variations and the sequences underlying these variations remain unknown. This study was aimed to address these issues by using a more general molecular marker, the randomly am- plified polymorphic DNA (RAPD), which is known to distribute and amplify unbiased loci throughout the genome (Sauve et al., 2005; Williams et al., 1990). We report that a high level of genomic variations indeed occurred in the R. ambigua var. idahoensis micro- propagated plants. Moreover, cellular genes and retroelements appeared to underlie a sub- stantial proportion of the variations.
基于应变测量的PC钢材料蠕变系数评估方法
DOI：
--
发表时间：
2013
期刊：
影响因子：
--
作者：
小川由布子;亀田昭一;佐藤良一;上谷康晴
通讯作者：
上谷康晴