Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp. conglutinans that Differ in Pathogenicity.
Comparative Proteomics Analyses of Two Races of Fusarium oxysporum f. sp. conglutinans that Differ in Pathogenicity.
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DOI：
10.1038/srep13663
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发表时间：
2015-09-03
影响因子：
4.6
通讯作者：
Xie B
中科院分区：
文献类型：
--
作者：
Li E;Ling J;Wang G;Xiao J;Yang Y;Mao Z;Wang X;Xie B
尖孢镰刀菌（Fusarium oxysporum）是一种土壤真菌，可在多种植物中引起血管性枯萎和根腐病。由两个小种组成的尖孢菌（F. oxysporum F. sp. conglutinans, Foc）可引起白菜枯萎病。与小种1（52557−TM, R1）相比，小种2（58385−TM, R2）表现出更强的致病性。本研究首次建立了Foc菌丝体和分生孢子的蛋白质组参考图谱，鉴定出145种不同丰度的蛋白。在这些蛋白中，R2菌丝体和分生孢子中的高丰度蛋白大部分参与碳水化合物、氨基酸和离子代谢，这表明这些蛋白可能在分离物R2较强的致病性中起重要作用。与蛋白质组学分析相比，20个典型基因的表达水平表现出类似的改变模式。选择参与碳水化合物代谢的蛋白葡聚糖基转移酶作为研究对象。我们敲除相应的基因（gas1），发现Foc-∆gas1与野生型分离株相比，显著降低了生长速度和毒力。这些结果加深了我们对白菜枯萎病致病力相关蛋白的认识，为白菜枯萎病的防治提供了新的契机。
Fusarium oxysporum is a soil-inhabiting fungus that induces vascular wilt and root rot in a variety of plants. F. oxysporum f. sp. conglutinans (Foc), which comprises two races, can cause wilt disease in cabbage. Compared with race 1 (52557−TM, R1), race 2 (58385−TM, R2) exhibits much stronger pathogenicity. Here, we provide the first proteome reference maps for Foc mycelium and conidia and identify 145 proteins with different abundances among the two races. Of these proteins, most of the high-abundance proteins in the R2 mycelium and conidia are involved in carbohydrate, amino acid and ion metabolism, which indicates that these proteins may play important roles in isolate R2’s stronger pathogenicity. The expression levels of 20 typical genes demonstrate similarly altered patterns compared to the proteomic analysis. The protein glucanosyltransferase, which is involved in carbohydrate metabolism, was selected for research. We knocked out the corresponding gene (gas1) and found that Foc-∆gas1 significantly reduced growth rate and virulence compared with wild type isolates. These results deepened our understanding of the proteins related to F. oxysporum pathogenicity in cabbage Fusarium wilt and provided new opportunities to control this disease.