Composition of Gut Microbiota of Children and Adolescents With Perinatal Human Immunodeficiency Virus Infection Taking Antiretroviral Therapy in Zimbabwe
Composition of Gut Microbiota of Children and Adolescents With Perinatal Human Immunodeficiency Virus Infection Taking Antiretroviral Therapy in Zimbabwe
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DOI：
10.1093/infdis/jiz473
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发表时间：
2020-02-01
影响因子：
6.4
通讯作者：
Flaegstad, Trond
中科院分区：
文献类型：
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作者：
Flygel, Trym T.;Sovershaeva, Evgeniya;Flaegstad, Trond
背景资料。人类免疫缺陷病毒(HIV)感染导致胃肠道屏障受损，肠道中的CD4(+)T细胞大量耗尽。抗逆转录病毒治疗(ART)可恢复CD4(+)计数，并可能对成年人的肠道微生物区系产生有益影响。关于长期抗逆转录病毒疗法对艾滋病毒感染儿童肠道微生物群的影响，人们知之甚少。我们调查了HIV感染和未感染儿童的肠道微生物区系组成，并评估了肠道微生物区系与患者特征之间的关系。在一项横断面研究中，从177名艾滋病毒感染者和103名未感染艾滋病毒的对照组收集了直肠拭子。用16S核糖体核糖核酸测序分析肠道微生物组成。感染人类免疫缺陷病毒的儿童与未感染艾滋病毒的儿童相比，其α多样性显著降低，而β多样性显著升高。未观察到微生物多样性与CD4(+)T细胞计数、HIV病毒载量或HIV相关慢性肺部疾病之间的关系。我们发现，在HIV感染的参与者中，棒状杆菌(P<；.01)、Finegoldia(P<；.01)和厌氧球菌(P<；.01)的水平较高，在CD4(+)计数较低的参与者中，肠杆菌科细菌(P=.02)的水平较高(P=.02)。
Background. Human immunodeficiency virus (HIV) infection causes impairment of the gastrointestinal barrier, with substantial depletion of CD4 (+) T cells in the gut. Antiretroviral therapy (ART) restores CD4 (+) counts and may have beneficial effects on gut microbiota in adults. Little is known about effect of long-term ART on gut microbiome in HIV-infected children. We investigated composition of gut microbiota in HIV-infected and -uninfected children and assessed associations between gut microbiota and patient characteristics.Methods. In a cross-sectional study, rectal swabs were collected from 177 HIV-infected and 103 HIV-uninfected controls. Gut microbial composition was explored using 16S ribosomal ribonucleic acid sequencing.Results. Human immunodeficiency virus-infected children had significantly lower alpha-diversity and higher beta-diversity compared to HIV-uninfected. No association was observed between microbiome diversity and CD4(+) T-cell count, HIV viral load, or HIV-associated chronic lung disease. We found enriched levels of Corynebacterium (P < .01), Finegoldia (P < .01), and Anaerococcus (P < .01) in HIV-infected participants and enrichment of Enterobacteriaceae (P = .02) in participants with low CD4(+) counts (