INCREASED EXPRESSION OF NEUTROPHIL AND MONOCYTE ADHESION MOLECULES IN UNSTABLE CORONARY-ARTERY DISEASE
INCREASED EXPRESSION OF NEUTROPHIL AND MONOCYTE ADHESION MOLECULES IN UNSTABLE CORONARY-ARTERY DISEASE
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DOI：
10.1161/01.cir.88.2.358
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发表时间：
1993-08-01
期刊：
影响因子：
37.8
通讯作者：
NOTARIO, A
中科院分区：
文献类型：
--
作者：
MAZZONE, A;DESERVI, S;NOTARIO, A
背景。白细胞粘附内皮细胞的快速增加是急性炎症反应和血管疾病发病机制中的首要事件之一。细胞表面糖蛋白的一个亚群（CD11/CD18复合物）在白细胞粘附过程中起主要作用；特别是，CD11b/CD18受体可以在趋化因子的作用下上调数倍。本研究的目的是评估具有缺血性心脏病临床表现的患者血液通过冠状动脉时，粒细胞和单核细胞CD11b/CD18受体的上调是否发生。方法与结果。对39例接受诊断性冠状动脉造影的患者进行了研究。1组（15例）临床诊断为不稳定型心绞痛，2组（14例）临床诊断为稳定型劳役型心绞痛，3组（10例）临床诊断为非典型胸痛。冠状动脉造影前，同时从冠状窦和主动脉取样。用直接免疫荧光法检测细胞表面受体，用带有荧光标记的单克隆抗体进行流式细胞荧光测定。白细胞在未分离的血液中染色，以避免可能激活吞噬细胞的体外操作。1组和2组患者有明显的冠状动脉疾病（至少有一条主要冠状动脉狭窄50%），而3组患者冠状动脉正常。在1组中，粒细胞和单核细胞在冠状窦中CD11b/CD18粘附受体的表达明显高于主动脉(P
Background. A rapid increase in leukocyte adhesion to endothelial cells is one of the first events in the acute inflammatory response and in the pathogenesis of vascular diseases. A subgroup of cell surface glycoproteins (the CD11/CD18 complex) play a major role in the leukocyte adhesion process; in particular, the CD11b/CD18 receptor can be upregulated severalfold in response to chemotactic factors. The purpose of this study was to assess whether upmodulation of granulocyte and monocyte CD11b/CD18 receptors takes place during the passage of blood through the coronary tree of patients with clinical manifestations of ischemic heart disease.Methods and Results. Thirty-nine patients who underwent diagnostic coronary arteriography were studied. Group 1 (15 patients) had a clinical diagnosis of unstable angina, group 2 (14 patients) had stable exertional angina, and group 3 (10 patients) had atypical chest pain. Simultaneous sampling from the coronary sinus and aorta was obtained before coronary arteriography. Cell surface receptors were detected by direct immunofluorescence evaluated by flow cytofluorimetry using monoclonal antibodies tagged with fluorescent markers. Leukocytes were stained in unseparated blood to avoid in vitro manipulation that could activate phagocytes. Group 1 and 2 patients had significant coronary artery disease (>50% coronary narrowing in at least one major coronary vessel), whereas group 3 patients had normal coronary arteries. In group 1, granulocytes and monocytes showed a significantly higher expression of the CD11b/CD18 adhesion receptor in the coronary sinus than in the aorta (both P