Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer
Retaining cell-cell contact enables preparation and culture of spheroids composed of pure primary cancer cells from colorectal cancer
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DOI：
10.1073/pnas.1015938108
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发表时间：
2011-04-12
影响因子：
11.1
通讯作者：
Inoue, Masahiro
中科院分区：
文献类型：
--
作者：
Kondo, Jumpei;Endo, Hiroko;Inoue, Masahiro
来自患者肿瘤的癌细胞的原代培养可以提供个体肿瘤的关键信息，但该技术迄今尚未优化。我们开发了一种创新的原代结直肠癌细胞培养方法，其原理是在整个过程中保持癌细胞的细胞-细胞接触。当肿瘤组织解离成细胞团时，其中细胞-细胞接触被保留，它们迅速形成球状体，我们称之为癌组织源性球状体（CTOS）。结肠直肠癌CTOS由高度纯化和活的癌细胞组成，并且它们以高效率制备。在免疫缺陷小鼠中，CTOS形成保留亲本肿瘤特征的异种移植肿瘤。此外，CTOS能够使用3D培养系统和干细胞培养基在体外培养和扩增。该方法允许评价来自个体患者的癌细胞中的化学敏感性和信号通路活化。简单的纯原代癌细胞制备和培养为研究癌症生物学和开发个性化药物提供了创新平台。
Primary culture of the cancer cells from patients' tumors can provide crucial information of individual tumors, yet the technology has not been optimized until now. We developed an innovative culture method for primary colorectal cancer cells, based on the principle that cell-cell contact of cancer cells was maintained throughout the process. When tumor tissue was dissociated into cell clusters, in which cell-cell contact was retained, they rapidly formed spheroids that we termed cancer tissue-originated spheroids (CTOSs). CTOSs of colorectal cancer consisted of highly purified and viable cancer cells, and they were prepared with high efficiency. In immunodeficient mice, CTOSs formed xenograft tumors that retained the features of the parental tumors. Moreover, CTOSs were able to be cultured and expanded in vitro using a 3D culture system and stem cell culture medium. This method allowed evaluation of chemosensitivity and signal pathway activation in cancer cells from individual patients. Easy preparation and culture of pure primary cancer cells provides an innovative platform for studying cancer biology and developing personalized medicine.